抗SOX17抗体(Anti-SOX17, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human SOX17Asp177-Val414Accession # Q9H6I2
Source	Monoclonal Mouse IgG3 Clone # 245013
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human SOX17 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human SOX2, 3, 7, or 10.

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Applications and Data

	Recommended Concentration	Sample
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

SOX17 in B16 Mouse Cell Line. SOX17 was detected in immersion fixed B16 mouse melanoma cell line using Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background	SOX17
background_content	Background: SOX17 SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

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Citations

1. PI3K/mTORC2 regulates TGF-beta/Activin signalling by modulating Smad2/3 activity via linker phosphorylation.
   Authors: Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, Cui W
   Nat Commun, 2015;6(0):7212.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
   Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
   Mol Ther, 2013;21(1):228-39.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells.
   Authors: Sui L, Mfopou J, Geens M, Sermon K, Bouwens L
   Biochem Biophys Res Commun, 2012;426(3):380-5.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Conditional gene expression in human embryonic stem cells.
   Authors: Vallier L, Alexander M, Pedersen R
   Stem Cells, 2007;25(6):1490-7.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. New cell lines from mouse epiblast share defining features with human embryonic stem cells.
   Authors: Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD
   Nature, 2007;448(7150):196-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
   Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
   FEBS Lett., 2006;580(25):5869-74.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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