抗MICA抗体(Anti-Human MICA Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	BaF3 mouse pro-B cell line transfected with human MICA
Source	Monoclonal Mouse IgG2B Clone # 159227
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human MICB.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of MICA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA PE‑conjugated Monoclonal Antibody (Catalog # FAB1300P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

MICA

background_content

Background: MICA MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

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Citations

1. Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma
   Authors: C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui
   Oncotarget, 2017;8(4):5954-5964.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. All-trans retinoic acid enhances cytotoxicity of CIK cells against human lung adenocarcinoma by upregulating MICA and IL-2 secretion
   Authors: XY Fan, PY Wang, C Zhang, YL Zhang, Y Fu, C Zhang, QX Li, JN Zhou, BE Shan, DW He
   Sci Rep, 2017;7(1):16481.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. SEP enhanced the antitumor activity of 5-fluorouracil by up-regulating NKG2D/MICA and reversed immune suppression via inhibiting ROS and caspase-3 in mice
   Authors: M Ke, H Wang, Y Zhou, J Li, Y Liu, M Zhang, J Dou, T Xi, B Shen, C Zhou
   Oncotarget, 2016;7(31):49509-49526.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow Cytometery


4. Increase of IFN-gamma and TNF-alpha production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor.
   Authors: Gomez-Lomeli P, Bravo-Cuellar A, Hernandez-Flores G, Jave-Suarez L, Aguilar-Lemarroy A, Lerma-Diaz J, Dominguez-Rodriguez J, Sanchez-Reyes K, Ortiz-Lazareno P
   Cancer Cell Int, 2014;14(1):100.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
   Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
   J Immunol, 2014;192(11):5332-42.
   Species: Human
   Sample Type: Whole Blood
   Application: Flow


6. HER2/HER3 signaling regulates NK cell-mediated cytotoxicity via MHC class I chain-related molecule A and B expression in human breast cancer cell lines.
   Authors: Okita R, Mougiakakos D, Ando T, Mao Y, Sarhan D, Wennerberg E, Seliger B, Lundqvist A, Mimura K, Kiessling R
   J. Immunol., 2012;188(5):2136-45.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. The MHC class Ib protein ULBP1 is a nonredundant determinant of leukemia/lymphoma susceptibility to gammadelta T-cell cytotoxicity.
   Authors: Lanca T, Correia DV, Moita CF, Raquel H, Neves-Costa A, Ferreira C, Ramalho JS, Barata JT, Moita LF, Gomes AQ, Silva-Santos B
   Blood, 2010;115(12):2407-11.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Expression of WSX1 in tumors sensitizes IL-27 signaling-independent natural killer cell surveillance.
   Authors: Dibra D, Cutrera JJ, Xia X, Birkenbach MP, Li S
   Cancer Res., 2009;69(13):5505-13.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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