抗IL-17 RC抗体(Anti-IL-17 RC, Goat-Poly antibody)
掲載日情報：2019/10/21 現在Webページ番号：31240
IL-17 RCに対する抗体(Anti-IL-17 RC, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||
|---|---|---|---|---|---|
|
Anti-IL-17 RC, Goat-Poly |
|
3 | |||
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Anti-Mouse IL-17 RC Affinity Purified Polyclonal Ab |
|
0 | |||
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IL-17 RC, Goat-Poly
文献数： 3
- 商品コード：AF2270
- メーカー：RSD
- 包装：100μg
- 価格：¥105,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Mouse IL-17 RC Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF2270-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RCLeu21-Trp465Accession # AAH04759 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse IL-17 RC in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-17 RC is observed and less than 5% cross-reactivity with recombinant mouse (rm) IL‑17 RD and rmIL-17B R is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse IL‑17 RC (Catalog # 2270-ML) |
| Flow Cytometry | 2.5 µg/106 cells | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Neutralization | Measured by its ability to neutralize IL‑17F-induced IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.6-3.6 µg/mL in the presence of 100 ng/mL Recombinant Human IL‑17F. | |
| Flow Cytometry | |
|---|---|
 | Detection of IL‑17 RC in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse IL‑17 RC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2270, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). |
| Neutralization | |
 | IL‑6 Secretion Induced by IL‑17F and Neutralization by Mouse IL‑17 RC Antibody. Recombinant Human IL‑17F (Catalog # 1335-INS) stimulates IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Human IL‑17F (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑17 RC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2270). The ND50 is typically 0.6-3.6 µg/mL. |
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Related Product & Information
| Long Name | Interleukin 17 Receptor C, Transcript Variant 3 |
|---|---|
| Entrez Gene IDs | 84818 (Human); 171095 (Mouse) |
| Background | IL-17 RC |
| background_content | Background: IL-17 RC IL-17 receptor C (IL-17 RC; also known as IL-17 RL) is an 85‑110 kDa member of the IL-17 receptor family. This is one of five families, termed IL-17 RA, B, C, D and E, that comprise the cytokine receptor superfamily (1‑6). Not all receptors appear to bind known members of the IL-17 cytokine family. To date, IL-17 RA is reported to bind IL-17A, while IL-17 RB is reported to bind IL-17B and IL-17E (2, 4). Mouse IL-17 RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. Full-length IL-17 RC is synthesized as a 674 amino acid (aa) precursor. It contains a 21 aa signal sequence, a 419 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 213 aa cytoplasmic region. There are multiple potential N‑linked glycosylation sites in the ECD and potential phosphorylation sites in the cytoplasmic tail. Four mouse variants have been identified that have been designated mIL-17 RC (7). The isoform expressed here as an R&D product is an unusual 567 aa form (8). Its precursor contains a 20 aa signal sequence, a 444 aa extracellular region, a 20 aa transmembrane segment and an 83 aa cytoplasmic tail. When compared to the full length mouse IL-17 RC form, this expressed isoform’s extracellular region shows absolute aa identity, save for an additional 24 aa insert. In the cytoplasmic region, it is highly divergent and shows virtually no aa identity (8‑9). The extracellular region of mouse IL-17 RC shows about 70% aa identity to the equivalent region in human IL-17 RC isoform # 3. IL-17 RC is the cognate receptor for IL-17F (7). In humans, IL-17 RC binds IL-17A with similar affinity, and with IL-17 RA, it forms a definitive receptor for both IL-17A and IL-17F (7). The stoichiometry is unclear; it may form a heterodimer with IL-17 RA, or a heterotrimer with a preexisting IL-17 RA homodimer (4, 7, 10, 11). The heteromeric nature of the receptor may be important given that the predominant form of the IL-17 cytokine is now considered to be an IL-17A:IL-17F heterodimer (4). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Respiratory syncytial virus infection of newborn CX3CR1-deficent mice induces a pathogenic pulmonary innate immune response
Authors: S Das, M Raundhal, J Chen, TB Oriss, R Huff, JV Williams, A Ray, P Ray
JCI Insight, 2017;2(17):.
Species: Mouse
Sample Type: In Vivo
Application: In vivo - Th17 cells mediate clade-specific, serotype-independent mucosal immunity.
Authors: Chen K, McAleer JP, Lin Y, Paterson DL, Zheng M, Alcorn JF, Weaver CT, Kolls JK
Immunity, 2012;35(6):997-1009.
Species: Mouse
Sample Type: In Vivo
Application: In vivo - TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses.
Authors: Nichols JR, Aldrich AL, Mariani MM, Vidlak D, Esen N, Kielian T
J. Immunol., 2009;182(11):7119-30.
Species: Mouse
Sample Type: Whole Cells
Application: Flow
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