抗Dectin-1抗体(Anti-Dectin-1, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7AThr66-Met201Accession # NP_072092
Source	Monoclonal Mouse IgG2B Clone # 259931
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	Human whole blood monocytes
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

Dectin‑1/CLEC7A in Human Dendritic Cells. Dectin‑1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin‑1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Entrez Gene IDs	64581 (Human); 56644 (Mouse)
Background	Dectin-1/CLEC7A
background_content	Background: Dectin-1/CLEC7A Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

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Citations

1. Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses
   Authors: MMM Kaisar, M Ritter, C Del Fresno, HS Jónasdótti, AJ van der Ha, LR Pelgrom, G Schramm, LE Layland, D Sancho, C Prazeres d, M Giera, M Yazdanbakh, B Everts
   PLoS Biol., 2018;16(4):e2005504.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. EphA2 is an epithelial cell pattern recognition receptor for fungal ?-glucans
   Authors: M Swidergall, NV Solis, MS Lionakis, SG Filler
   Nat Microbiol, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysate
   Application: WB


3. Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells
   Authors: CC Yeh, HC Horng, H Chou, HY Tai, HD Shen, SL Hsieh, PH Wang
   Int J Mol Sci, 2017;18(3):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
   PLoS ONE, 2016;11(11):e0167410.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.
   Authors: Gazendam R, van Hamme J, Tool A, Hoogenboezem M, van den Berg J, Prins J, Vitkov L, van de Veerdonk F, van den Berg T, Roos D, Kuijpers T
   J Immunol, 2016;196(3):1272-83.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.
   Authors: Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li X, Hori T, Zurawski S, Liu Y, Zurawski G, Oh S
   J Immunol, 2015;195(4):1723-31.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response.
   Authors: Elder M, Webster S, Williams D, Gaston J, Goodall J
   Eur J Immunol, 2015;46(2):455-63.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
   Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
   PLoS ONE, 2015;10(3):e0120948.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


9. Podosomes of dendritic cells facilitate antigen sampling.
   Authors: Baranov, Maksim V, Ter Beest, Martin, Reinieren-Beeren, Inge, Cambi, Alessand, Figdor, Carl G, van den Bogaart, Geert
   J Cell Sci, 2014;127(0):1052-64.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis.
    Authors: Rodrigues D, Fernandes R, Balderramas H, Penitenti M, Bachiega T, Calvi S, Dias-Melicio L, Ikoma M, Soares A
    Cytokine, 2014;69(1):102-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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