抗GFAP抗体(Anti-GFAP, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human GFAPLeu292-Met432Accession # P14136
Source	Monoclonal Mouse IgG1 Clone # 273807
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human GFAP in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	50 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below

Western Blot
	Detection of Human GFAP by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	GFAP in Rat Cortical Stem Cells. GFAP was detected in immersion fixed differentiated rat cortical stem cells using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Simple Western
	Detection of Human GFAP by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Glial Fibrillary Acidic Protein
Background	GFAP
background_content	Background: GFAP Glial Fibrillary Acidic Protein (GFAP) is the predominant component of astrocyte intermediate filaments in the central nervous system. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous systems.

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Citations

1. MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain.
   Authors: Zeng, L, He, X, Wang, Y, Tang, Y, Zheng, C, Cai, H, Liu, J, Wang, Y, Fu, Y, Yang, G-Y
   Gene Ther, 2014;21(1):37-43.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-OCT embedded


2. Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility.
   Authors: Cruz-Orengo L, Daniels B, Dorsey D, Basak S, Grajales-Reyes J, McCandless E, Piccio L, Schmidt R, Cross A, Crosby S, Klein R
   J Clin Invest, 2014;124(6):2571-84.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Not specified


3. Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.
   Authors: Mangiola, Annunzia, Saulnier, Nathalie, De Bonis, Pasquale, Orteschi, Daniela, Sica, Gigliola, Lama, Gina, Pettorini, Benedett, Sabatino, Giovanni, Zollino, Marcella, Lauriola, Libero, Colabianchi, Anna, Proietti, Gabriell, Kovacs, Gyula, Maira, Giulio, Anile, Carmelo
   PLoS ONE, 2013;8(3):e57145.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


4. CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres.
   Authors: Walter D, Satheesha S, Albrecht P, Bornhauser BC, D'Alessandro V, Oesch SM, Rehrauer H, Leuschner I, Koscielniak E, Gengler C, Moch H, Bernasconi M, Niggli FK, Schafer BW
   PLoS ONE, 2011;6(5):e19506.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Presence of pluripotent CD133+ cells correlates with malignancy of gliomas.
   Authors: Thon N, Damianoff K, Hegermann J, Grau S, Krebs B, Schnell O, Tonn JC, Goldbrunner R
   Mol. Cell. Neurosci., 2010;43(1):51-9.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Development of a culture system that supports adult microglial cell proliferation and maintenance in the resting state.
   Authors: Ponomarev ED, Novikova M, Maresz K, Shriver LP, Dittel BN
   J. Immunol. Methods, 2005;300(1):32-46.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC

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