抗Vimentin抗体(Anti-Vimentin, Human, Rat-Mono antibody)
掲載日情報：2018/11/26 現在Webページ番号：31069
Vimentinに対する抗体(Anti-Vimentin, Human, Rat-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年04月26日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Vimentin, Human, Rat-Mono(280618) |
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Anti-Human/Mouse/Rat Vimentin MAb (Clone 280618) |
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[在庫・価格 ：2025年04月26日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Vimentin, Human, Rat-Mono(280618)
文献数： 9
- 商品コード：MAB2105
- メーカー：RSD
- 包装：100μg
- 価格：¥103,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：FLJ36605 クローン：280618 Genbank No： 7431 Protein Accession No： P08670 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Rat | ||||
| 交差性 | Human | 適用 | FCM,IC,IHC,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗神経幹細胞マーカー抗体 |
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Anti-Human/Mouse/Rat Vimentin MAb (Clone 280618)
文献数： 4
- 商品コード：MAB2105-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human, Mouse, Rat |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human VimentinSer2-Glu466Accession # P08670 |
| Source | Monoclonal Rat IgG2A Clone # 280618 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Vimentin antibodies are ideal for immunocytochemistry colocalization studies in intermediate filaments. Detects human, mouse and rat Vimentin in Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1-2 µg/mL | See below |
| Simple Western | 10 µg/mL | See below |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Western Blot | |
|---|---|
 | Detection of Human Vimentin by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Western Blot | |
 | Detection of Mouse and Rat Vimentin by Western Blot. Western blot shows lysates of MEF mouse embryonic feeder cells, NIH‑3T3 mouse embryonic fibroblast cell line, Rat‑2 rat embryonic fibroblast cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | Vimentin in NTera‑2 Human Cell Line. Vimentin was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunocytochemistry | |
 | Vimentin in A549 Human Cell Line. Vimentin was detected in immersion fixed A549 human lung carcinoma cell line using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunocytochemistry | |
 | Vimentin in Mouse Cortical Stem Cells. Vimentin was detected in immersion fixed mouse cortical stem cells using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunocytochemistry | |
 | Vimentin in Rat Cortical Stem Cells. Vimentin was detected in immersion fixed rat cortical stem cells using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Simple Western | |
 | Detection of Human Vimentin by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at approximately 58 kDa (as indicated) using 10 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
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Related Product & Information
| Background | Vimentin |
|---|---|
| background_content | Background: Vimentin Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G
Nat Cell Biol, 2015;17(9):1193-204.
Species: Human
Sample Type: Whole Tissue
Application: IHC - Not specified - GLI1, CTNNB1 and NOTCH1 protein expression in a thymic epithelial malignancy tissue microarray.
Authors: Riess J, West R, Dean M, Klimowicz A, Neal J, Hoang C, Wakelee H
, 2015;0(0):.
Species: Human
Sample Type: Whole Tissue
Application: IHC - Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures.
Authors: Vrana NE, Builles N, Justin V, Bednarz J, Pellegrini G, Ferrari B, Damour O, Hulmes DJ, Hasirci V
Invest. Ophthalmol. Vis. Sci., 2008;49(12):5325-31.
Species: Human
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
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