抗Mouse LYVE-1抗体(Anti-Mouse LYVE-1 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	BaF/3 mouse pro-B cell line transfected with mouse LYVE-1Ala24-Thr234Accession # Q8BHC0
Source	Monoclonal Rat IgG2A Clone # 223322
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.02-0.1 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Hyaluronan to immobilized Recombinant Mouse LYVE-1 (Catalog # 2125-LY) coated at 5 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	Immersion fixed LYVE-1 transfected cells

Western Blot
	Detection of Mouse LYVE‑1 by Western Blot. Western blot shows lysate of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LYVE‑1 Monoclonal Antibody (Catalog # MAB2125) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LYVE‑1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of LYVE‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE‑1 Monoclonal Antibody (Catalog # MAB2125, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

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Related Product & Information

Long Name	Lymphatic Vessel Endothelial Hyaluronan Receptor 1
Entrez Gene IDs	10894 (Human); 114332 (Mouse); 293186 (Rat)
Background	LYVE-1
background_content	Background: LYVE-1 Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity. LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

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Citations

1. Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program
   Authors: M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen
   Nat Commun, 2018;9(1):1511.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. In vivo photolabeling of tumor-infiltrating cells reveals highly regulated egress of T-cell subsets from tumors
   Authors: T Torcellan, HR Hampton, J Bailey, M Tomura, R Brink, T Chtanova
   Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh


3. Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1
   Authors: LA Johnson, S Banerji, W Lawrance, U Gileadi, G Prota, KA Holder, YM Roshorm, T Hanke, V Cerundolo, NW Gale, DG Jackson
   Nat. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib
   Authors: Tianliang Sun
   J. Cell Biol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration
   Authors: Tsun-Ho Lo
   J Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


6. A micro-sterile inflammation array as an adjuvant for influenza vaccines.
   Authors: Wang J, Shah D, Chen X, Anderson R, Wu M
   Nat Commun, 2014;5(0):4447.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


7. Non-invasive mapping of deep-tissue lymph nodes in live animals using a multimodal PET/MRI nanoparticle.
   Authors: Thorek D, Ulmert D, Diop N, Lupu M, Doran M, Huang R, Abou D, Larson S, Grimm J
   Nat Commun, 2014;5(0):3097.
   


8. Low molecular weight hyaluronan induces lymphangiogenesis through LYVE-1-mediated signaling pathways.
   Authors: Wu M, Du Y, Liu Y, He Y, Yang C, Wang W, Gao F
   PLoS ONE, 2014;9(3):e92857.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


9. Fusing VE-cadherin to alpha-catenin impairs fetal liver hematopoiesis and lymph but not blood vessel formation.
   Authors: Dartsch N, Schulte D, Hagerling R, Kiefer F, Vestweber D
   Mol Cell Biol, 2014;34(9):1634-48.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


10. A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes.
    Authors: Reichardt, Peter, Patzak, Irene, Jones, Kristian, Etemire, Eloho, Gunzer, Matthias, Hogg, Nancy
    EMBO J, 2013;32(6):829-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF

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