抗Nestin抗体(Anti-Nestin, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	NS0 mouse myeloma cell line transfected with human Nestin
Source	Monoclonal Mouse IgG1 Clone # 196908
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Nestin.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either untreated (dark orange filled histogram) or treated with 5 μM Retinoic Acid for 60 days (light orange filled histogram) was stained with Mouse Anti-Human Nestin PE‑conjugated Monoclonal Antibody (Catalog # IC1259P) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

目次に戻る

Related Product & Information

Background

Nestin

background_content

Background: Nestin Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

目次に戻る

Citations

1. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
   Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
   Oncotarget, 2017;8(17):28074-28092.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Phenotypic diversity of patient-derived melanoma populations in stem cell medium.
   Authors: Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M
   Lab Invest, 2015;95(6):672-83.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Expression of the stem cell markers nestin and CD133 on circulating melanoma cells.
   Authors: Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U
   J. Invest. Dermatol., 2011;131(2):487-94.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. MDR1 expression identifies human melanoma stem cells.
   Authors: Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G
   Biochem. Biophys. Res. Commun., 2008;368(4):930-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

目次に戻る