抗Flk-1抗体(Anti-Flk-1, Phosphorylated, Rabbit-Poly antibody)
掲載日情報：2018/11/26 現在Webページ番号：30770
Flk-1に対する抗体(Anti-Flk-1, Phosphorylated, Rabbit-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年06月08日 13時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||
|---|---|---|---|---|---|
|
Anti-Flk-1(pTyr1214), Phosphorylated, Rabbit-Poly <Anti-VEGF Receptor 2> <Anti-KDR> |
|
6 | |||
|
Anti-Phospho-VEGF R2/KDR (Y1214) Affinity Purified Polyclonal Ab |
|
0 | |||
[在庫・価格 ：2024年06月08日 13時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Flk-1(pTyr1214), Phosphorylated, Rabbit-Poly <Anti-VEGF Receptor 2> <Anti-KDR>
文献数： 6
- 商品コード：AF1766
- メーカー：RSD
- 包装：50μg
- 価格：¥85,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Phospho-VEGF R2/KDR (Y1214) Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF1766-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Phosphopeptide containing human VEGF R2/KDR/Flk-1 Y1214 site |
| Source | Polyclonal Rabbit IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human VEGF R2/KDR/Flk‑1 in Western blots when phosphorylated at Y1214. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.5 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Intracellular Staining by Flow Cytometry | 2.5 µg/106 cells | See below |
| Western Blot | |
|---|---|
 | Detection of Human Phospho-VEGF R2/KDR/Flk‑1 (Y1214) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-VEGF R2/KDR/Flk‑1 (Y1214) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-VEGF R2/KDR/Flk‑1 (Y1214) at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | Phospho-VEGF R2/KDR/Flk-1 (Y1214) in A431 Human Cell Line. VEGF R2/KDR/Flk‑1 phosphorylated at Y1214 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-VEGF R2/KDR/Flk‑1 (Y1214) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1766) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of Phospho-VEGF R2/KDR/Flk‑1 in pervanadate-treated HUVEC by Flow Cytometry. HUVEC human umbilical vein endothelial cells were unstimulated (yellow filled histogram) or treated with 100 μM pervanadate for 15 minutes (orange filled histogram), then stained with Rabbit Anti-Human Phospho‑VEGF R2/KDR/Flk‑1(Y1214) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1766), or control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. |
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Related Product & Information
| Background | VEGF R2/KDR/Flk-1 |
|---|---|
| background_content | Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGF R2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Endothelial progenitor cells transplantation attenuated blood-brain barrier damage after ischemia in diabetic mice via HIF-1?
Authors: J Geng, L Wang, M Qu, Y Song, X Lin, Y Chen, M Mamtilahun, S Chen, Z Zhang, Y Wang, GY Yang
Stem Cell Res Ther, 2017;8(1):163.
Species: Human
Sample Type: Whole Cells
Application: ICC - DSGOST inhibits tumor growth by blocking VEGF/VEGFR2-activated angiogenesis
Oncotarget, 2016;7(16):21775-85.
Species: Human
Sample Type: Cell Lysates
Application: WB - Fluorescent resonance energy transfer imaging of VEGFR dimerization.
Authors: Ahmadova Z, Yagublu V, Forg T, Hajiyeva Y, Jesenofsky R, Hafner M, Keese M
Anticancer Res, 2014;34(5):2123-33.
Species: Hamster
Sample Type: Whole Cells
Application: ICC - Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro.
Br. J. Cancer, 2012;107(10):1702-13.
Species: Human
Sample Type: Cell Lysates
Application: TaqMan Protein Assay
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