抗HIF-1α抗体(Anti-HIF-1α, Human/Mouse/Rat, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human HIF-1 alpha Arg575-Asn826Accession # Q16665.1
Source	Monoclonal Mouse IgG1 Clone # 241809
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human, mouse, and rat HIF-1 alpha.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunoprecipitation	1-3 µg/500 µg cell lysate	MCF‑7 human breast cancer cell line treated with CoCl2, see our available Western blot detection antibodies
Knockout Validated	HIF‑1 alpha is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HIF‑1 alpha knockout HeLa cell line.

Western Blot
	Detection of Human, Mouse, and Rat HIF‑1 alpha by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Balb‑3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 μM CoCl2 for 8 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF‑1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Human HIF‑1 alpha by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF‑1 alpha at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Knockout Validated
	Western Blot Shows Human HIF‑1 alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO overnight. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF‑1 alpha at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Knockout Validated
	HIF‑1 alpha Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HIF‑1 alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF‑1 alpha knockout (KO) HeLa Human Cell Line cell line using Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Long Name	Hypoxia-inducible Transcription Factor 1 alpha
Background	HIF-1 alpha
background_content	Background: HIF-1 alpha The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

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Citations

1. Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip
   Authors: S Jalili-Fir, R Prantil-Ba, A Jiang, R Potla, T Mammoto, JC Weaver, TC Ferrante, HJ Kim, JMS Cabral, O Levy, DE Ingber
   Cell Death Dis, 2018;9(2):223.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Silencing HIF-1? induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells
   Authors: AP Fischer, SL Miles
   Biochem. Biophys. Res. Commun., 2017;0(0):.
   Species: Human
   Sample Type: Protein
   Application: WB


3. Protective effect of zinc preconditioning against renal ischemia reperfusion injury is dose dependent
   Authors: K Rao, K Sethi, J Ischia, L Gibson, L Galea, L Xiao, M Yim, M Chang, N Papa, D Bolton, A Shulkes, GS Baldwin, O Patel
   PLoS ONE, 2017;12(7):e0180028.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia
   Authors: P Hernansanz, E Ramos, E Navarro, E Parada, N Sánchez-Ló, L Peláez-Agu, JD Cabrera-Ga, D Tello, I Buendia, A Marina, J Egea, MG López, A Bogdanova, A Martínez-R
   Redox Biol, 2017;12(0):1040-1051.
   Species: Bovine
   Sample Type: Cell Lysates
   Application: WB


5. Ferrochelatase is a therapeutic target for ocular neovascularization
   Authors: HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson
   EMBO Mol Med, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: Alleviation by pantethine
   Authors: M van Gijsel, K Baranger, P Benech, S Rivera, M Khrestchat, M de Reggi, B Gharib
   PLoS ONE, 2017;12(4):e0175369.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


7. XIAP upregulates expression of HIF target genes by targeting HIF1? for Lys63-linked polyubiquitination
   Authors: CV Park, IG Ivanova, NS Kenneth
   Nucleic Acids Res., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
   Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
   Oncotarget, 2017;8(17):28074-28092.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency
   Authors: M Sone, N Morone, T Nakamura, A Tanaka, K Okita, K Woltjen, M Nakagawa, JE Heuser, Y Yamada, S Yamanaka, T Yamamoto
   Cell Metab., 2017;25(5):1103-1117.e6.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


10. VHL promotes immune response against renal cell carcinoma via NF-?B-dependent regulation of VCAM-1
    Authors: D Labrousse-, E Martínez-A, M Corral-Esc, R Bienes-Mar, J Berridy, L Serrano-Ov, E Conde, ML García-Ber, JM Giménez-Ba, AS Salinas-Sá, R Sánchez-Pr, M Yao, M Lasa, MJ Calzada
    J. Cell Biol, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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