抗Galectin-3 Binding Protein/Mac-2 Binding Protein抗体(Anti-Galectin-3 Binding Protein/Mac-2 Binding Protein, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Galectin‑3BP/MAC‑2BPVal19-Asp585Accession # Q08380
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Galectin‑3BP/MAC‑2BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately15% cross‑reactivity with recombinant mouse MAC‑2BP is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunocytochemistry	1-15 µg/mL	See below

Western Blot
	Detection of HumanGalectin‑3BP/MAC‑2BP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	Galectin‑3BP/MAC‑2BP in HeLa Human Cell Line. Galectin‑3BP/MAC‑2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
	Detection of HumanGalectin‑3BP/MAC‑2BP by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Background

Galectin-3BP/MAC-2BP

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Background: Galectin-3BP/MAC-2BP Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.

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Citations

1. Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
   Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
   J. Clin. Invest, 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. Lectin, galactoside-binding soluble 3 binding protein promotes 17-N-Allylamino-17-demethoxygeldanamycin resistance through PI3K/Akt pathway in lung cancer cell line
   Authors: JK Woo, JE Jang, JH Kang, JK Seong, YS Yoon, HC Kim, SJ Lee, SH Oh
   Mol. Cancer Ther, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner
   Sci Rep, 2016;6(0):32736.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6.
   J. Virol., 2012;86(12):6620-31.
   Species: Human
   Sample Type: Serum
   Application: WB


5. A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
   Authors: Silverman A, Nakata R, Shimada H, Sposto R, DeClerck Y
   Cancer Res, 2012;72(9):2228-38.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
   Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
   Exp. Cell Res., 2011;317(7):976-93.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma.
   Authors: Fukaya Y, Shimada H, Wang LC, Zandi E, DeClerck YA
   J. Biol. Chem., 2008;283(27):18573-81.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB

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