抗VE-Cadherin抗体(Anti-VE-Cadherin, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human VE-CadherinAsp48-Gln593Accession # P33151
Source	Monoclonal Mouse IgG2B Clone # 123413
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin andno cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	HUVEC human umbilical vein endothelial cells stained in buffer containing Ca2+ and Mg2+
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	0.5-25 µg/mL	See below

Western Blot
	Detection of Human VE‑Cadherin by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VE‑Cadherin Monoclonal Antibody (Catalog # MAB9381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VE‑Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	VE‑Cadherin in HUVEC Cells. VE‑Cadherin was detected in immersion fixed HUVEC cells using Mouse Anti-HumanVE‑Cadherin Monoclonal Antibody (Catalog # MAB9381) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

VE-Cadherin

background_content

Background: VE-Cadherin Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74% homology.

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Citations

1. A Three-Dimensional Cell Culture System To Model RNA Virus Infections at the Blood-Brain Barrier
   Authors: JC Bramley, CG Drummond, NJ Lennemann, CA Good, KS Kim, CB Coyne
   mSphere, 2017;2(3):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function
   Authors: K Kulhankova, KJ Kinney, JM Stach, FA Gourronc, IM Grumbach, AJ Klingelhut, W Salgado-Pa
   Infect. Immun., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
   Authors: Gabriela Odent Grig
   J Tissue Eng Regen Med, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
   Authors: Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM
   Cell Stem Cell, 2008;3(1):55-68.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
   Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P
   Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H610-9.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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