抗E-Cadherin抗体(Anti-E-Cadherin, Mouse, Rat-Mono antibody)
掲載日情報：2018/11/26 現在Webページ番号：30470
E-Cadherinに対する抗体(Anti-E-Cadherin, Mouse, Rat-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年06月25日 17時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-E-Cadherin, Mouse, Rat-Mono(114420) |
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Anti-Mouse E-Cadherin MAb (Clone 114420) |
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[在庫・価格 ：2025年06月25日 17時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-E-Cadherin, Mouse, Rat-Mono(114420)
文献数： 8
- 商品コード：MAB7481
- メーカー：RSD
- 包装：100μg
- 価格：¥94,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：Arc-1 クローン：114420 Genbank No： 999 Protein Accession No： P09803 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Rat | ||||
| 交差性 | Mouse | 適用 | FCM,IC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗胚性幹細胞マーカー抗体 |
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Anti-Mouse E-Cadherin MAb (Clone 114420)
文献数： 0
- 商品コード：MAB7481-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse E-CadherinAsp157-Val709 (predicted)Accession # P09803 |
| Source | Monoclonal Rat IgG2A Clone # 114420 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse E-Cadherin in direct ELISAs and Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 2 µg/mL | See below |
| Flow Cytometry | 0.25 µg/106 cells | D3 mouse embryonic stem cell line stained in buffer containing Ca2+ and Mg2+ |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 8-25 µg/mL | Immersion fixed D3 mouse embryonic stem cell line |
| Western Blot | |
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 | Detection of Mouse E‑Cadherin by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse E‑Cadherin Monoclonal Antibody (Catalog # MAB7481) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for E‑Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Related Product & Information
| Entrez Gene IDs | 999 (Human); 12550 (Mouse); 83502 (Rat) |
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| Background | E-Cadherin |
| background_content | Background: E-Cadherin Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. A soluble form of E-Cadherin can be released from epithelial cell surfaces by proteolysis. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination with Cell Polarization During Ameloblast Differentiation
J Bone Miner Res, 2016;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: ICC - Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties
Sci Rep, 2016;6(0):37914.
Species: Mouse
Sample Type: Whole Cells
Application: Neut - The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland.
Authors: Bittencourt F, Wu S, Bridges J, Miller W
J Virol, 2014;88(20):11811-24.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Fresh - Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
Authors: Zhang, Hanwei, Chin, Arnold I
PLoS ONE, 2014;9(4):e94793.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - Integrin alpha9beta1 in airway smooth muscle suppresses exaggerated airway narrowing.
J. Clin. Invest., 2012;122(8):2916-27.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Frozen - E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
Authors: Moore RN, Cherry JF, Mathur V
Stem Cells Dev., 2012;21(1):30-41.
Species: Mouse
Sample Type: Whole Cells
Application: ICC
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