抗STAT3抗体(Anti-STAT3, Human/Mouse/Rat, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human STAT3Met1-Asn175Accession # P40763
Source	Monoclonal Mouse IgG2B Clone # 232209
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human, mouse, and rat STAT3 in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunoprecipitation	1-3 µg/500 µg cell lysate	Daudi human Burkitt's lymphoma cell line, see our available Western blot detection antibodies
Immunocytochemistry	3-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below
Knockout Validated	STAT3 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT3 knockout HeLa cell line.

Intracellular Staining by Flow Cytometry
	Detection of STAT3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Western Blot
	Detection of Human STAT3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT3 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	STAT3 in HeLa Human Cell Line. STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Knockout Validated
	STAT3 Specificity is Shown by Immunocytochemistry in KnockoutCell Line. STAT3 was detected in immersion fixed HeLa human cervicalepithelial carcinoma cell line treated with IFN-alpha 1, but is not detected inSTAT3 knockout (KO) HeLa cell line using Mouse Anti-Human/Mouse/Rat STAT3Monoclonal Antibody (Catalog # MAB1799) at 1 µg/mL for 3 hours at roomtemperature. Cells were stained using the NorthernLights™ 557-conjugatedAnti-Mouse IgG Secondary Antibody (red; Catalog # NL007)and counterstained with DAPI (blue). Specific staining was localized tocytoplasm and nuclei. View our protocol for FluorescentICC Staining of Cells on Coverslips.
Knockout Validated
	Western Blot Shows Human STAT3 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, STAT6 KO HeLa cell line,. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STAT3 at approximately 80 kDa (as indicated) in the parental HeLacell line, but is not detectable in the STAT3 knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Signal Transducer and Activator of Transcription 3
Background	STAT3
background_content	Background: STAT3 Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. Janus kinases (JAKs) phosphorylate STAT proteins and induce dimerization. Homo- or heterodimers translocate to the nucleus where they bind to DNA and activate transcription.

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Citations

1. Tumor-derived mesenchymal-stem-cell-secreted IL-6 enhances resistance to cisplatin via the STAT3 pathway in breast cancer
   Authors: H Xu, Y Zhou, W Li, B Zhang, H Zhang, S Zhao, P Zheng, H Wu, J Yang
   Oncol Lett, 2018;15(6):9142-9150.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Inhibition of STAT3 Signaling Reduces IgA1 Autoantigen Production in IgA Nephropathy
   Authors: K Yamada, ZQ Huang, M Raska, C Reily, JC Anderson, H Suzuki, H Ueda, Z Moldoveanu, K Kiryluk, Y Suzuki, RJ Wyatt, Y Tomino, AG Gharavi, A Weinmann, BA Julian, CD Willey, J Novak
   Kidney Int Rep, 2017;2(6):1194-1207.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Proangiogenic tumor proteins as potential predictive or prognostic biomarkers for bevacizumab therapy in metastatic colorectal cancer.
   Authors: Bruhn M, Townsend A, Khoon Lee C, Shivasami A, Price T, Wrin J, Arentz G, Tebbutt N, Hocking C, Cunningham D, Hardingham J
   Int J Cancer, 2014;135(3):731-41.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


4. IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
   Authors: Kolliputi N, Waxman AB
   Am. J. Respir. Cell Mol. Biol., 2009;40(3):314-24.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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