抗SOX2抗体(Anti-SOX2, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	E. coli-derived recombinant human SOX2Gly135-Met317Accession # P48431
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects SOX2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX17 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human SOX2
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry

SOX2 in Human iPSK3 cells. SOX2 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Goat Anti-Human SOX2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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Related Product & Information

Background	SOX2
background_content	Background: SOX2 SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

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Citations

1. Eva1 Maintains the Stem-like Character of Glioblastoma-Initiating Cells by Activating the Noncanonical NF-?B Signaling Pathway
   Authors: N Ohtsu, Y Nakatani, D Yamashita, S Ohue, T Ohnishi, T Kondo
   Cancer Res., 2016;76(1):171-81.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. Conversion of differentiated cancer cells into cancer stem-like cells in a glioblastoma model after primary chemotherapy.
   Authors: Auffinger B, Tobias A, Han Y, Lee G, Guo D, Dey M, Lesniak M, Ahmed A
   Cell Death Differ, 2014;21(7):1119-31.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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