抗Human GITR/TNFRSF18 Fluorescein抗体(Anti-Human GITR/TNFRSF18 Fluorescein antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18Gln26-Glu161 (Thr43Ala)Accession # Q9Y5U5
Source	Monoclonal Mouse IgG1 Clone # 110416
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse (rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of GITR/TNFRSF18 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 1 μg/mL PHA for 24 hours were stained with Mouse Anti-Human GITR/TNFRSF18 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB689F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

GITR/TNFRSF18

background_content

Background: GITR/TNFRSF18 GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up‑regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

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Citations

1. Stem cell source-dependent reconstitution of FOXP3+ T cells after pediatric SCT and the association with allo-reactive disease.
   Authors: Reubsaet, L L, de Pagter, A P J, van Baarle, D, Keukens, L, Nanlohy, N, Sanders, E A M, Prakken, B J, Boelens, J J, de Kleer, I M
   Bone Marrow Transplant, 2013;48(4):502-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
   Authors: Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
   PLoS ONE, 2011;6(1):e15997.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Generation and accumulation of immunosuppressive adenosine by human CD4+CD25highFOXP3+ regulatory T cells.
   Authors: Mandapathil M, Hilldorfer B, Szczepanski MJ, Czystowska M, Szajnik M, Ren J, Lang S, Jackson EK, Gorelik E, Whiteside TL
   J. Biol. Chem., 2010;285(10):7176-86.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. CD4+CD25high T cell numbers are enriched in the peripheral blood of patients with rheumatoid arthritis.
   Authors: Han GM, O'Neil-Andersen NJ, Zurier RB, Lawrence DA
   Cell. Immunol., 2008;253(1):92-101.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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