抗Human OX40 Ligand/TNFSF4抗体(Anti-Human OX40 Ligand/TNFSF4 antibody)
掲載日情報：2018/11/26 現在Webページ番号：30242
Human OX40 Ligand/TNFSF4に対する抗体(Anti-Human OX40 Ligand/TNFSF4 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年07月01日 18時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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Anti-Human OX40 Ligand/TNFSF4 MAb (Clone 159403) |
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Anti-Human OX40 Ligand/TNFSF4 MAb (Clone 159403) |
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Anti-Human OX40 Ligand/TNFSF4 MAb (Clone 159403) |
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0 | |||
[在庫・価格 ：2025年07月01日 18時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human OX40 Ligand/TNFSF4 MAb (Clone 159403)
文献数： 0
- 商品コード：MAB10541-100
- メーカー：RSD
- 包装：100μg
- 価格：¥79,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human OX40 Ligand/TNFSF4 MAb (Clone 159403)
文献数： 0
- 商品コード：MAB10541-500
- メーカー：RSD
- 包装：500μg
- 価格：¥266,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human OX40 Ligand/TNFSF4 MAb (Clone 159403)
文献数： 0
- 商品コード：MAB10541-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥27,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human OX40 LigandGln51-Leu183Accession # P23510 |
| Source | Monoclonal Mouse IgG1 Clone # 159403 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human OX40 Ligand in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhCD27 Ligand, recombinant mouse (rm) CD27 Ligand, rhCD30 Ligand, rmCD30 Ligand, rhCD40 Ligand, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, recombinant cotton rat TNF-alpha, rhTNF-alpha, rmTNF-alpha, recombinant porcine TNF-alpha, recombinant rat TNF-alpha, rhTRAIL, rhTRANCE, rmTRANCE, or rhVEGF is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Flow Cytometry | 2.5 µg/106 cells | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Neutralization | Measured by its ability to neutralize OX40 Ligand/TNFSF4-induced IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40. Muller, N. et al. (2008) FEBS Journal 275:2296. The Neutralization Dose (ND50) is typically 0.1-0.6 μg/mL in the presence of 10 ng/mL Recombinant Human OX40 Ligand/TNFSF4. | |
| Flow Cytometry | |
|---|---|
 | Detection of OX40 Ligand/TNFSF4 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with 20 ng/mL Recombinant Human IL‑4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 7 days and 1 μg/mL LPS, 20 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) for last 24 hours were stained with Mouse Anti-Human OX40 Ligand/TNFSF4 Monoclonal Antibody(Catalog # MAB10541, filled histogram) or isotype control (Catalog # MAB002, open histogram), followed by Goat F(ab)2 Anti-Mouse IgG (H+L) Allophycocyanin (Catalog # F0101B). |
| Immunocytochemistry | |
 | OX40 Ligand/TNFSF4 in Human PBMCs. OX40 Ligand/TNFSF4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS using Mouse Anti-Human OX40 Ligand/TNFSF4 Monoclonal Antibody (Catalog # MAB10541) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (orange; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Neutralization | |
 | IL‑8 Secretion Induced by OX40 Ligand/TNFSF4 and Neutralization by Human OX40 Ligand/TNFSF4 Antibody. Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) induces IL‑8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40 in a dose-dependent manner (orange line) as measured by Human IL‑8 Duoset (Catalog # DY208). IL‑8 Secretion elicited by Recombinant Human OX40 Ligand/TNFSF4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human OX40 Ligand/TNFSF4 Monoclonal Antibody (Catalog # MAB10541). The ND50 is typically 0.1-0.6 μg/mL. |
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Related Product & Information
| Entrez Gene IDs | 7292 (Human); 22164 (Mouse); 89814 (Rat) |
|---|---|
| Background | OX40 Ligand/TNFSF4 |
| background_content | Background: OX40 Ligand/TNFSF4 OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein designated TNFSF4 within the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1-23) and a carboxy-terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Like other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the group of co-stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4-1BB Ligand. OX40 appears as a late activation-induced T cell surface antigen, and its major function of OX40-OX40L interaction may be to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses
Authors: MMM Kaisar, M Ritter, C Del Fresno, HS Jónasdótti, AJ van der Ha, LR Pelgrom, G Schramm, LE Layland, D Sancho, C Prazeres d, M Giera, M Yazdanbakh, B Everts
PLoS Biol., 2018;16(4):e2005504.
Species: Human
Sample Type: Whole Cells
Application: Neut - Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling.
Authors: Beynon V, Quintana FJ, Weiner HL
PLoS ONE, 2012;7(6):e39576.
Species: Human
Sample Type: Whole Cells
Application: Neut - B7-CD28 costimulatory signals control the survival and proliferation of murine and human gammadelta T cells via IL-2 production.
J. Immunol., 2012;189(3):1202-8.
Species: Human
Sample Type: Whole Cells
Application: Neut - Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
Blood, 2005;105(2):750-8.
Species: Human
Sample Type: Cell Culture Supernates
Application: Neut
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