抗Human B7-1/CD80 Fluorescein抗体(Anti-Human B7-1/CD80 Fluorescein antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80Val35-Asn242 (predicted)Accession # P33681
Source	Monoclonal Mouse IgG1 Clone # 37711
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of B7‑1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB140F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

B7-1/CD80

background_content

Background: B7-1/CD80 B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through Interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequence identity. Human and mouse B7-1 share 44% aa sequence identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

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Citations

1. Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors
   Authors: KP Bene, DW Kavanaugh, C Leclaire, AP Gunning, DA MacKenzie, A Wittmann, ID Young, N Kawasaki, E Rajnavolgy, N Juge
   Front Microbiol, 2017;8(0):321.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors.
   Authors: Petterson T, Jendholm J, Mansson A, Bjartell A, Riesbeck K, Cardell LO
   J. Leukoc. Biol., 2011;89(2):177-87.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. NKG2D-dependent effector function of bronchial epithelium-activated alloreactive T-cells.
   Authors: Kraetzel K, Stoelcker B, Eissner G, Multhoff G, Pfeifer M, Holler E, Schulz C
   Eur. Respir. J., 2008;32(3):563-70.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates.
   Authors: Kaur K, Chowdhury S, Greenspan NS, Schreiber JR
   Blood, 2007;110(8):2948-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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