抗Mer抗体(Anti-Mer, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human MerArg26-Ala499Accession # Q12866
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Mer and less than 1% cross-reactivity with recombinant human (rh) Axl and rhDtk is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	Human peripheral blood monocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot

Detection of Human Mer by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and PANC‑1 human pancreatic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Mer at approximately 170-230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

目次に戻る

Related Product & Information

Background

Mer

background_content

Background: Mer Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

目次に戻る

Citations

1. Human Sertoli cells support high levels of Zika virus replication and persistence
   Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
   Sci Rep, 2018;8(1):5477.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses
   Authors: AS Richard, BS Shim, YC Kwon, R Zhang, Y Otsuka, K Schmitt, F Berri, MS Diamond, H Choe
   Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):2024-2029.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation
   Authors: SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


4. Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
   Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
   Br. J. Cancer, 2017;117(12):1787-1797.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


6. IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.
   Authors: Zizzo G, Cohen P
   J Immunol, 2013;190(10):5237-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
   Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
   J Immunol, 2012;189(7):3508-20.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
   J. Exp. Med., 2012;209(11):2033-47.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Ectosomes released by polymorphonuclear neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages.
   Authors: Eken C, Martin PJ, Sadallah S, Treves S, Schaller M, Schifferli JA
   J. Biol. Chem., 2010;285(51):39914-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
    Authors: Uehara H, Shacter E
    J. Immunol., 2008;180(4):2522-30.
    Species: Human
    Sample Type: Cell Lysates
    Application: Neut

目次に戻る