抗IgG 二次抗体(Anti-IgG, Rabbit, Goat-Poly, HRP antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rabbit
Label	HRP (Horseradish Peroxidase)
Immunogen	Rabbit IgG
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	Use at a 1:1000 dilution.

Western Blot
	Detection of RabbitIgG Primary Antibody by Western Blot Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60Polyclonal Antibody (Catalog # AF1800) using a 1:1000 dilution ofHRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008).  This experiment was conducted under reducingconditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Immunoglobulin G
Background	IgG
background_content	Background: IgG R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

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Citations

1. Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3
   Authors: L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner
   PLoS ONE, 2018;13(5):e0195427.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


2. Key Proteins Involved in Spheroid Formation and Angiogenesis in Endothelial Cells After Long-Term Exposure to Simulated Microgravity
   Authors: A Dittrich, D Grimm, J Sahana, J Bauer, M Krüger, M Infanger, NE Magnusson
   Cell. Physiol. Biochem., 2018;45(2):429-445.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Development of matrix metalloproteinase-targeted probes for lung inflammation detection with positron emission tomography
   Authors: N Kondo, T Temma, K Aita, S Shimochi, K Koshino, M Senda, H Iida
   Sci Rep, 2018;8(1):1347.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


4. Low-dose paclitaxel downregulates MYC proto-oncogene bHLH transcription factor expression in colorectal carcinoma cells
   Authors: W Li, W Zhu, C Lv, H Qu, K Xu, H Li, H Li, Y Du, G Liu, Y Wang, HJ Wei, HY Zhao
   Oncol Lett, 2018;15(2):1881-1887.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. The Role of Diacylglycerol Acyltransferase (DGAT) 1 and 2 in Cardiac Metabolism and Function
   Authors: ND Roe, MK Handzlik, T Li, R Tian
   Sci Rep, 2018;8(1):4983.
   Application: WB


6. Antioxidant nitroxides protect hepatic cells from oxidative stress-induced cell death
   Authors: S Shinto, Y Matsuoka, M Yamato, KI Yamada
   J Clin Biochem Nutr, 2018;62(2):132-138.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


7. Polymorphism of Receptor-Type Tyrosine-Protein Phosphatase Delta gene in the development of non-alcoholic fatty liver disease
   Authors: S Nakajima, H Tanaka, K Sawada, H Hayashi, T Hasebe, M Abe, C Hasebe, M Fujiya, T Okumura
   J. Gastroenterol. Hepatol., 2017;0(0):.
   Species: Human
   Sample Type: Protein
   Application: IHC


8. Antioxidant Treatment Induces Hyperactivation of the HPA Axis by Upregulating ACTH Receptor in the Adrenal and Downregulating Glucocorticoid Receptors in the Pituitary
   Authors: JP Prevatto, RC Torres, BL Diaz, PMRE Silva, MA Martins, VF Carvalho
   Oxid Med Cell Longev, 2017;2017(0):4156361.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


9. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
   Authors: KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis
   Nat. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: WB


10. Antiangiogenic effects of catalpol on rat corneal�neovascularization
    Authors: Y Han, M Shen, LY Tang, G Tan, QC Yang, L Ye, LH Ye, N Jiang, GP Gao, Y Shao
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB

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