抗Human ABCG2抗体(Anti-Human ABCG2 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	3T3 cells transduced with human ABCG2
Source	Monoclonal Mouse IgG2B Clone # 5D3
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human ABCG2 in flow cytometry and immunocytochemistry.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	Human ABCG2 transfected CHO-S cells
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry
	ABCG2 in JAR Human Cell Line. ATP-Binding Cassette Transporter G2 (ABCG2) was detected in immersion fixed JAR human choriocarcinoma cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	ABCG2 in Human ABCG2 transfected CHO Chinese Hamster Cell Line. ABCG2 was detected in immersion fixed Human ABCG2 transfected CHO Chinese hamster ovary cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

ABCG2

background_content

Background: ABCG2 Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34- cells and is downregulated with the acquisition of CD34 on the cell surface (6).

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Citations

1. In�Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling
   Authors: K Yamamizu, M Iwasaki, H Takakubo, T Sakamoto, T Ikuno, M Miyoshi, T Kondo, Y Nakao, M Nakagawa, H Inoue, JK Yamashita
   Stem Cell Reports, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. The Metastatic Potential and Chemoresistance of Human Pancreatic Cancer Stem Cells.
   Authors: Bhagwandin V, Bishop J, Wright W, Shay J
   PLoS ONE, 2015;11(2):e0148807.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Mitochondrial localization of ABC transporter ABCG2 and its function in 5-aminolevulinic acid-mediated protoporphyrin IX accumulation.
   Authors: Kobuchi, Hirotsug, Moriya, Koko, Ogino, Tetsuya, Fujita, Hirofumi, Inoue, Keiji, Shuin, Taro, Yasuda, Tatsuji, Utsumi, Kozo, Utsumi, Toshihik
   PLoS ONE, 2012;7(11):e50082.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Human umbilical cord blood-derived non-hematopoietic stem cells suppress lymphocyte proliferation and CD4, CD8 expression.
   Authors: Ji F, Wang Y, Sun H, Du J, Zhao H, Wang D, Xu Q, Duan D, Yang H
   J. Neuroimmunol., 2008;197(2):99-109.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. CD molecules 2006--human cell differentiation molecules.
   Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
   J. Immunol. Methods, 2007;319(1):1-5.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea.
   Authors: Shimmura S, Nagoshi N, Matsuzaki Y, Okano H, Tsubota K
   Stem Cells, 2006;24(12):2714-22.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC

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