抗Sca-1抗体(Anti-Sca-1, Mouse, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E alleleLeu27-Gly119Accession # CAA28351
Source	Monoclonal Rat IgG2A Clone # 177228
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse Sca‑1/Ly6 in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE‑conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca‑1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Immunocytochemistry
	Sca‑1/Ly6 in Mouse Splenocytes. Sca‑1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse Sca‑1/Ly6 Monoclonal Antibody (Catalog # MAB1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background

Sca-1/Ly6

background_content

Background: Sca-1/Ly6 Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

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Citations

1. Multicolor quantitative confocal imaging cytometry
   Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
   Nat. Methods, 2018;15(1):39-46.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Tissue-engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel.
   Authors: Hibino N, Villalona G, Pietris N
   FASEB J., 2011;25(8):2731-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. BCL6 is critical for the development of a diverse primary B cell repertoire.
   Authors: Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jack HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alboran IM, Melnick AM, Ye BH, Muschen M
   J. Exp. Med., 2010;207(6):1209-21.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Magnetic surface-enhanced Raman spectroscopic (M-SERS) dots for the identification of bronchioalveolar stem cells in normal and lung cancer mice.
   Authors: Noh MS, Jun BH, Kim S, Kang H, Woo MA, Minai-Tehrani A, Kim JE, Kim J, Park J, Lim HT, Park SC, Hyeon T, Kim YK, Jeong DH, Lee YS, Cho MH
   Biomaterials, 2009;30(23):3915-25.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Intranasal HGF administration ameliorates the physiologic and morphologic changes in lung emphysema.
   Authors: Hegab AE, Kubo H, Yamaya M, Asada M, He M, Fujino N, Mizuno S, Nakamura T
   Mol. Ther., 2008;16(8):1417-26.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen

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