抗β-Tubulin抗体(Anti-β-Tubulin, Neuronal Class Ⅲ, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Label	Unconjugated
Immunogen	Rat brain-derived microtubules
Source	Monoclonal Mouse IgG2A Clone # TuJ-1
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below

Western Blot
	Detection of Human and Mouse beta ‑III Tubulin by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta ‑III Tubulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	beta ‑III Tubulin in Differentiated Human Neural Progenitor Cells. beta ‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	beta ‑III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes. beta ‑III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained forbeta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the NorthernLights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells. beta ‑III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) and 10 µg/mL Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
	Detection of Rat beta ‑III Tubulin by Simple WesternTM. Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Entrez Gene IDs	10381 (Human)
Background	beta-III Tubulin
background_content	Background: beta-III Tubulin beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

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Citations

1. Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction
   Authors: JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman
   Mol Ther Methods Clin Dev, 2018;9(0):172-180.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Multicolor quantitative confocal imaging cytometry
   Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
   Nat. Methods, 2018;15(1):39-46.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus
   Authors: K Abdi, CH Lai, P Paez-Gonza, M Lay, J Pyun, CT Kuo
   Nat Commun, 2018;9(1):1655.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Generation of multidrug resistant human tissues by overexpression of the ABCG2 multidrug transporter in embryonic stem cells
   Authors: Z Erdei, A Schamberge, G Török, K Szebényi, G Várady, TI Orbán, L Homolya, B Sarkadi, Á Apáti
   PLoS ONE, 2018;13(4):e0194925.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement
   Authors: E Emmerson, AJ May, L Berthoin, N Cruz-Pache, S Nathan, AJ Mattingly, JL Chang, WR Ryan, AD Tward, SM Knox
   EMBO Mol Med, 2018;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


6. Nitrosative damage during retrovirus infection-induced neuropathic pain
   Authors: P Chauhan, WS Sheng, S Hu, S Prasad, JR Lokensgard
   J Neuroinflammation, 2018;15(1):66.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Regulation of neuritogenesis in hippocampal neurons using stiffness of extracellular microenvironment
   Authors: A Tanaka, Y Fujii, N Kasai, T Okajima, H Nakashima
   PLoS ONE, 2018;13(2):e0191928.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


8. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
   Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
   Cell Rep, 2017;19(1):20-35.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation
   Authors: J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski
   Cell Rep, 2017;19(2):281-294.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Transfer of pathogenic and nonpathogenic cytosolic proteins between spinal cord motor neurons in vivo in chimeric mice
    Authors: EV Thomas, WA Fenton, J McGrath, AL Horwich
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC

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