抗Gas 6抗体(Anti-Gas 6, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Gas6Asp118-Ala678Accession # NP_000811
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Gas6 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse Gas6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Gas6 (Catalog # 885-GS)

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Related Product & Information

Background

Gas6

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Background: Gas6 Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% amino acid (aa) sequence identity with mouse and rat Gas6 and 43% aa sequence identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF-like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF‑induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

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Citations

1. IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.
   Authors: Zizzo G, Cohen P
   J Immunol, 2013;190(10):5237-46.
   Species: Human
   Sample Type: Cell Culture Supernates
   


2. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
   Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
   J Immunol, 2012;189(7):3508-20.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


3. Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
   Authors: Hung YJ, Lee CH, Chu NF
   Diabetes Care, 2010;33(8):1840-4.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


4. Gas6 evaluation in patients with acute dyspnea due to suspected pulmonary embolism.
   Authors: Sainaghi PP, Alciato F, Carnieletto S, Castello L, Bergamasco L, Sola D, Bongo AS, Inglese E, Polosa R, Avanzi GC
   Respir Med, 2009;103(4):589-94.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


5. Development of a novel immunoassay for the assessment of plasma Gas6 concentrations and their variation with hormonal status.
   Authors: Clauser S, Peyrard S, Gaussem P, Crespin M, Emmerich J, Aiach M, Borgel D
   Clin. Chem., 2007;53(10):1808-13.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development

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