抗6-His抗体(Anti-6-His, Mouse-Mono, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Label	Biotin
Immunogen	His-tagged peptide
Source	Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects proteins containing accessible consecutive histidine regions. This antibody detects His tags localized at the amino- or carboxyl-terminus.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below

Western Blot

Detection of His Tag by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-His Tag Biotinylated Monoclonal Antibody (Catalog # BAM050) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Histidine Tag
Background	His Tag
background_content	Background: His Tag Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

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Citations

1. Antibodies Targeting Closely Adjacent or Minimally Overlapping Epitopes Can Displace One Another
   Authors: YN Abdiche, AY Yeung, I Ni, D Stone, A Miles, W Morishige, A Rossi, P Strop
   PLoS ONE, 2017;12(1):e0169535.
   Species: Human
   Sample Type: Recombinant Protein
   Application: ELISA Development


2. High-throughput epitope binning assays on label-free array-based biosensors can yield exquisite epitope discrimination that facilitates the selection of monoclonal antibodies with functional activity.
   Authors: Abdiche, Yasmina, Miles, Adam, Eckman, Josh, Foletti, Davide, Van Blarcom, Thomas J, Yeung, Yik Andy, Pons, Jaume, Rajpal, Arvind
   PLoS ONE, 2014;9(3):e92451.
   Species: N/A
   Sample Type: Protein
   Application: Bioassay


3. Designed ankyrin repeat proteins: a new approach to mimic complex antigens for diagnostic purposes?
   Authors: Hausammann, Stefanie, Vogel, Monique, Kremer Hovinga, Johanna, Lacroix-Desmazes, Sebastie, Stadler, Beda M, Horn, Michael
   PLoS ONE, 2013;8(4):e60688.
   Species: Human
   Sample Type:
   Application: Binding Assay


4. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies.
   Authors: Fransson J, Tornberg UC, Borrebaeck CA, Carlsson R, Frendeus B
   Int. J. Cancer, 2006;119(2):349-58.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen

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