抗MDM2抗体(Anti-MDM2, Human/Mouse/Rat, Rabbit-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human MDM2 aa 3-491
Source	Polyclonal Rabbit IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat MDM2.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry
	MDM2 in Mouse Kidney. MDM2 was detected in perfusion fixed frozen sections of mouse kidney using Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Western Blot
	Detection of Human MDM2 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, MCF‑7 human breast cancer cell line, and CEM human T‑lymphoblastoid cell line untreated (-) or treated (+) with 5 μM LLnV for 1 hour. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MDM2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Double Minute 2 Protein
Background	MDM2/HDM2
background_content	Background: MDM2/HDM2 MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally up‑regulates the expression of its own inhibitor, MDM2.

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Citations

1. Multicolor quantitative confocal imaging cytometry
   Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
   Nat. Methods, 2018;15(1):39-46.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Adverse Effects on ?-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function
   Authors: R Schreckenb, P Bencsik, M Weber, Y Abdallah, C Csonka, K Gömöri, K Kiss, J Pálóczi, J Pipis, M Sárközy, P Ferdinandy, R Schulz, KD Schlüter
   J Am Heart Assoc, 2017;6(12):.
   Species: Rat
   Sample Type: Tissue Homogenate
   Application: WB


3. Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo.
   Authors: Phesse, T J, Myant, K B, Cole, A M, Ridgway, R A, Pearson, H, Muncan, V, van den Brink, G R, Vousden, K H, Sears, R, Vassilev, L T, Clarke, A R, Sansom, O J
   Cell Death Differ, 2014;21(6):956-66.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


4. HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.
   J Hematol Oncol, 2012;5(0):57.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.
   Authors: Sosin, Angela M, Burger, Angelika, Siddiqi, Aisha, Abrams, Judith, Mohammad, Ramzi M, Al-Katib, Ayad M
   J Hematol Oncol, 2012;5(0):57.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Cell death via DR5, but not DR4, is regulated by p53 in myeloma cells.
   Authors: Surget S, Chiron D, Gomez-Bougie P, Descamps G, Menoret E, Bataille R, Moreau P, Le Gouill S, Amiot M, Pellat-Deceunynck C
   Cancer Res, 2012;72(17):4562-73.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. A high-frequency regulatory polymorphism in the p53 pathway accelerates tumor development.
   Authors: Post SM, Quintas-Cardama A, Pant V, Iwakuma T, Hamir A, Jackson JG, Maccio DR, Bond GL, Johnson DG, Levine AJ, Lozano G
   Cancer Cell, 2010;18(3):220-30.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation.
   Authors: Rozan LM, McDonald ER, Navaraj A, Matthew EM, Dicker DT, El-Deiry WS
   J. Biol. Chem., 2007;282(5):3273-81.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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