抗p53抗体(Anti-p53, Phosphorylated, Rabbit-Poly antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Phosphopeptide containing human p53 S15 site
Source	Polyclonal Rabbit IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, monkey, and rat p53 when phosphorylated at S15 and mouse p53 when phosphorylated at S18.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunoprecipitation	1 µg/500 µg cell lysate	CEM human T-lymphoblastoid cell line exposed to UV-C and MCF‑7 human breast cancer cell line treated with camptothecin (CPT), see our available Western blot detection antibodies
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human Phospho-p53 (S15) by Western Blot. Western blot shows lysates of CEM human T-lymphoblastoid cell line untreated or exposed to 100 J/m2 UV-C for the indicated time. PVDF membrane was probed with 0.2 µg/mL Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S15) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. For additional reference, duplicate lysates were probed with 1:5000 dilution Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	Phospho-p53 (S15) in HeLa Human Cell Line. p53 phosphorylated at S15 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with UV light (left panel) or untreated (right panel) using Rabbit Anti-Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using Rat Anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	Phospho-p53 (S15) in Human Breast Cancer Tissue. p53 phosphorylated at S15 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Immunohistochemistry
	Phospho-p53 (S15) in Human Breast Cancer Tissue. p53 phosphorylated at S15 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

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Related Product & Information

Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
Background	p53
background_content	Background: p53 The p53 tumor suppressor protein is a multi-functional transcription factor that regulates proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Human p53 is a 393 amino acid (aa) nuclear/cytoplasmic protein that contains an N-terminal activation domain, a specific DNA binding domain, and a C-terminal domain that mediates tetramer formation and regulates DNA binding. P53 is phosphorylated on Ser15 in response to irradiation, and this phosphorylation increases recruitment of the coactivator CREB-binding protein/p300. The ATM or ATR kinases can phosphorylate p53 at serine 15 (S15), which leads to cell cycle arrest. Serine 15 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.

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Citations

1. Skeletal muscle DNA damage precedes spinal motor neuron DNA damage in a mouse model of Spinal Muscular Atrophy (SMA).
   Authors: Fayzullina, Saniya, Martin, Lee J
   PLoS ONE, 2014;9(3):e93329.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


2. Noninvasive assessment of regulable transferred-p53 gene expression and evaluation of therapeutic response with FDG-PET in tumor model.
   Authors: Aung W, Hasegawa S, Koshikawa-Yano M
   Gene Ther., 2010;17(9):1142-51.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. The p53 oncoprotein is a substrate for tissue transglutaminase kinase activity.
   Authors: Mishra S, Murphy LJ
   Biochem. Biophys. Res. Commun., 2005;339(2):726-30.
   Species: Human
   Sample Type: Recombinant Protein
   Application: WB

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