抗Sca-1抗体(Anti-Sca-1, Mouse, Rat-Mono, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E alleleLeu27-Gly119Accession # CAA28351
Source	Monoclonal Rat IgG2A Clone # 177228
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse Sca-1/Ly6 in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse Sca‑1/Ly6 under non-reducing conditions only
Flow Cytometry	2.5 µg/106 cells	Mouse splenocytes
Immunohistochemistry	8-25 µg/mL	See below

Immunohistochemistry
	Sca‑1/Ly6 in Mouse Kidney. Sca‑1/Ly6 was detected in perfusion fixed frozen sections of mouse kidney using Mouse Sca‑1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	Sca‑1/Ly6 in Mouse Thymus. Sca‑1/Ly6 was detected in perfusion fixed frozen sections of mouse thymus using Mouse Sca‑1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

Sca-1/Ly6

background_content

Background: Sca-1/Ly6 Sca-1 is an 18 kDa phosphatidylositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2,3). Sca-1-positive HSC can be found in the adult bone marrow, fetal liver, and mobilized peripheral blood and spleen in the adult animal (2-7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9-12).

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Citations

1. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
   Authors: S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen
   Cell Stem Cell, 2018;22(1):35-49.e7.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Allergen exposure-induced differences in CD34+ cell phenotype: relationship to eosinophilopoietic responses in different compartments.
   Authors: Sergejeva S, Johansson AK, Malmhall C, Lotvall J
   Blood, 2004;103(4):1270-7.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC

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