抗Human E-Cadherin抗体(Anti-Human E-Cadherin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human E-CadherinAsp155-Ile707Accession # P12830
Source	Monoclonal Mouse IgG2B Clone # 180215
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human E-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cadherin-8, rhCadherin-17, recombinant mouse E-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE‑Cadherin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunohistochemistry	2-25 µg/mL	See below

Western Blot
	Detection of Human E‑Cadherin by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB1838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E‑Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	E-Cadherin in Human Colon. E-Cadherin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) at 2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human E‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 166 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB1838). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background

E-Cadherin

background_content

Background: E-Cadherin Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, andL-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E-Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

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Citations

1. Modeling of Aniridia-Related Keratopathy by CRISPR/Cas9 Genome Editing of Human Limbal Epithelial Cells and Rescue by Recombinant PAX6 Protein
   Authors: LN Roux, I Petit, R Domart, JP Concordet, J Qu, H Zhou, A Joliot, O Ferrigno, D Aberdam
   Stem Cells, 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Rose Bengal acetate photodynamic therapy (RBAc-PDT) induces exposure and release of Damage-Associated Molecular Patterns (DAMPs) in human HeLa cells.
   Authors: Panzarini, Elisa, Inguscio, Valentin, Fimia, Gian Mar, Dini, Luciana
   PLoS ONE, 2014;9(8):e105778.
   Species: Human
   Sample Type: Cell Fraction
   Application: WB


3. Progression of oral squamous cell carcinoma accompanied with reduced e-cadherin expression but not cadherin switch.
   Authors: Hashimoto T, Soeno Y, Maeda G, Taya Y, Aoba T, Nasu M, Kawashiri S, Imai K
   PLoS ONE, 2012;7(10):e47899.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


4. Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems.
   Authors: Maupin KA, Sinha A, Eugster E, Miller J, Ross J, Paulino V, Keshamouni VG, Tran N, Berens M, Webb C, Haab BB
   PLoS ONE, 2010;5(9):e13002.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
   Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
   Cancer Res., 2009;69(15):6249-55.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Vascular endothelial growth factor-A stimulates Snail expression in breast tumor cells: implications for tumor progression.
   Authors: Wanami LS, Chen HY, Peiro S, Garcia de Herreros A, Bachelder RE
   Exp. Cell Res., 2008;314(13):2448-53.
   Species: Human
   Sample Type: Whole Cells
   Application: WB


7. Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity.
   Authors: Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K
   Cancer Res., 2004;64(6):2024-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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