抗Caspase-3抗体(Anti-Caspase-3, Cleaved, Rabbit-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	KLH-conjugated human Caspase-3 synthetic peptideCRGTELDCGIETDAccession # U26943
Source	Monoclonal Rabbit IgG Clone # 269518
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below

Western Blot
	Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase‑3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cleaved Caspase‑3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Intracellular Staining by Flow Cytometry
	Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (open histogram) or  treated with 1 μM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase‑3 Monoclonal Antibody (Catalog # MAB835, filled histogram) followed by anti-Rabbit IgG FITC-conjugated secondary antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.
Immunocytochemistry
	Caspase‑3 in Jurkat Human Cell Line. Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with staurosporin using Human/Mouse Cleaved Caspase‑3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Entrez Gene IDs	836 (Human); 12367 (Mouse)
Background	Caspase-3
background_content	Background: Caspase-3 Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

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Citations

1. Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis
   Authors: BS Moon, J Bai, M Cai, C Liu, J Shi, W Lu
   Nat Commun, 2018;9(1):401.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Formyl peptide receptor 1 suppresses gastric cancer angiogenesis and growth by exploiting inflammation resolution pathways
   Authors: N Prevete, F Liotti, A Illiano, A Amoresano, P Pucci, A de Paulis, RM Melillo
   Oncoimmunology, 2017;6(4):e1293213.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


3. Loss of Tmem30a leads to photoreceptor degeneration
   Authors: L Zhang, Y Yang, S Li, S Zhang, X Zhu, Z Tai, M Yang, Y Liu, X Guo, B Chen, Z Jiang, F Lu, X Zhu
   Sci Rep, 2017;7(1):9296.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
   Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
   Cell Death Dis, 2017;8(7):e2955.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
   Authors: F Sallustio, C Curci, A Aloisi, CC Toma, E Marulli, G Serino, SN Cox, G De Palma, A Stasi, C Divella, R Rinaldi, FP Schena
   Sci Rep, 2017;7(1):8225.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. The protective effects of Resveratrol against radiation-induced intestinal injury
   Authors: H Zhang, H Yan, X Zhou, H Wang, Y Yang, J Zhang, H Wang
   BMC Complement Altern Med, 2017;17(1):410.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


7. Potent Vasoconstrictor Kisspeptin-10 Induces Atherosclerotic Plaque Progression and Instability: Reversal by its Receptor GPR54 Antagonist
   Authors: K Sato, R Shirai, M Hontani, R Shinooka, A Hasegawa, T Kichise, T Yamashita, H Yoshizawa, R Watanabe, TA Matsuyama, H Ishibashi-, S Koba, Y Kobayashi, T Hirano, T Watanabe
   J Am Heart Assoc, 2017;6(4):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Resveratrol fuels HER2 and ER?-positive breast cancer behaving as proteasome inhibitor
   Authors: C Andreani, C Bartolacci, K Wijnant, R Crinelli, M Bianchi, M Magnani, A Hysi, M Iezzi, A Amici, C Marchini
   Aging (Albany NY), 2017;9(2):508-523.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


9. Melatonin alleviates lipopolysaccharide-compromised integrity of blood-brain barrier through activating AMP-activated protein kinase in old mice
   Authors: X Wang, GX Xue, WC Liu, H Shu, M Wang, Y Sun, X Liu, YE Sun, CF Liu, J Liu, W Liu, X Jin
   Aging Cell, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


10. H-Ras and K-Ras oncoproteins induce different tumor spectra when driven by the same regulatory sequences
    Cancer Res., 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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