抗GITR Ligand/TNFSF18抗体(Anti-GITR Ligand/TNFSF18, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GITR Ligand/TNFSF18Glu52-Ser177Accession # Q9UNG2
Source	Monoclonal Mouse IgG1 Clone # 109101
Purification	Protein A or G purified from ascites
Specificity	Detects human GITR Ligand/TNFSF18 in direct ELISAs.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	HUVEC human umbilical vein endothelial cells
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.02-0.06 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Human GITR Ligand/TNFSF18 to immobilized Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR) coated at 2 µg/mL (100 µL/well). At 0.3 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

目次に戻る

Related Product & Information

Long Name	Glucocorticoid Induced TNF Receptor Family Related Gene Ligand
Entrez Gene IDs	8995 (Human); 240873 (Mouse); 364031 (Rat)
Background	GITR Ligand/TNFSF18
background_content	Background: GITR Ligand/TNFSF18 GITR (glucocorticoid-induced TNF receptor superfamily-related protein, also named AITR, activation-inducible TNF receptor superfamily-related protein) and GITR ligand (GITRL) are novel members of the TNF receptor (TNFR) and TNF superfamilies (SF) that have been designated TNFRSF18 and TNFSF18, respectively. Human GITRL cDNA encodes a 177 amino acid residues type II membrane protein. The carboxy-terminal extracellular domain shows sequence identity to TNF/TNFSF2 (21%), Fas ligand/TNFSF6 (21%), TRAIL/TNFSF10 (18%), and lymphotoxin alpha /TNFSF1 (18%). GITRL is constitutively expressed in human umbilical vein endothelial cells but is not expressed in resting or stimulated T cell lines, B cell lines or peripheral blood mononuclear cells. GITR, the receptor for GITRL, is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up-regulated by antigen-receptor stimulation or by treatment with soluble anti-CD3 plus anti-CD28 or PMA plus ionomycin. Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2 and protect cells from TCR activation-induced cell death. It has been proposed that GITRL and GITR may modulate T lymphocyte functions in peripheral tissues.

目次に戻る

Citations

1. Generation and preclinical characterization of a Fc-optimized GITR-Ig fusion protein for induction of NK cell reactivity against leukemia.
   Authors: Schmiedel, Benjamin, Werner, Antje, Steinbacher, Julia, Nuebling, Tina, Buechele, Corina, Grosse-Hovest, Ludger, Salih, Helmut R
   Mol Ther, 2013;21(4):877-86.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Neutralization of tumor-derived soluble glucocorticoid-induced TNFR-related protein ligand increases NK cell anti-tumor reactivity.
   Authors: Baltz KM, Krusch M, Baessler T, Schmiedel BJ, Bringmann A, Brossart P, Salih HR
   Blood, 2008;112(9):3735-43.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


3. Cancer immunoediting by GITR (glucocorticoid-induced TNF-related protein) ligand in humans: NK cell/tumor cell interactions.
   Authors: Baltz KM, Krusch M, Bringmann A, Brossart P, Mayer F, Kloss M, Baessler T, Kumbier I, Peterfi A, Kupka S, Kroeber S, Menzel D, Radsak MP, Rammensee HG, Salih HR
   FASEB J., 2007;21(10):2442-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Staphylococcal enterotoxin B inhibits regulatory T cells by inducing glucocorticoid-induced TNF receptor-related protein ligand on monocytes.
   Authors: Cardona ID, Goleva E, Ou LS, Leung DY
   J. Allergy Clin. Immunol., 2006;117(3):688-95.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL).
   Authors: Hanabuchi S, Watanabe N, Wang YH, Ito T, Shaw J, Cao W, Qin FX, Liu YJ
   Blood, 2006;107(9):3617-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
   Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
   Blood, 2005;105(2):750-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Neut

目次に戻る