抗Podocalyxin抗体(Anti-Podocalyxin, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human PodocalyxinSer23-Arg427Accession # AAB61574
Source	Monoclonal Mouse IgG2A Clone # 222328
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human Podocalyxin
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
	Podocalyxin in BG01V Human Stem Cells. Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

Podocalyxin

background_content

Background: Podocalyxin Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

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Citations

1. Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells
   Authors: E Tamayo-Orb, L Amo, M Riñón, N Nieto, E Amutio, N Maruri, M Solaun, A Arrieta, S Larrucea
   Oncotarget, 2017;8(59):99722-99739.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Self-organization of the human embryo in the absence of maternal tissues
   Nat. Cell Biol., 2016;18(6):700-8.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Not Specified


3. Podocalyxin-like protein is expressed in glioblastoma multiforme stem-like cells and is associated with poor outcome.
   Authors: Binder Z, Siu I, Eberhart C, ap Rhys C, Bai R, Staedtke V, Zhang H, Smoll N, Piantadosi S, Piccirillo S, Dimeco F, Weingart J, Vescovi A, Olivi A, Riggins G, Gallia G
   PLoS ONE, 2013;8(10):e75945.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - paraffin


4. Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury.
   Authors: Angelotti ML, Ronconi E, Ballerini L, Peired A, Mazzinghi B, Sagrinati C, Parente E, Gacci M, Carini M, Rotondi M, Fogo AB, Lazzeri E, Lasagni L, Romagnani P
   Stem Cells, 2012;30(8):1714-25.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.
   Authors: Lasagni L, Ballerini L, Angelotti ML
   Stem Cells, 2010;28(9):1674-85.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


6. Regeneration of glomerular podocytes by human renal progenitors.
   Authors: Ronconi E, Sagrinati C, Angelotti ML
   J. Am. Soc. Nephrol., 2009;20(2):322-32.
   Species: Human
   Sample Type: Whole Cells
   Application: cell sorting

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