抗Serum Albumin抗体(Anti-Serum Albumin, Human/Mouse, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Human Serum Albumin
Source	Monoclonal Mouse IgG2A Clone # 188835
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Serum Albumin in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2-1 µg/mL	See below
Simple Western	1-10 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below

Western Blot
	Detection of Human, Mouse, and Rat Albumin by Western Blot. Western blot shows lysates of human plasma, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot
	Detection of Human Albumin by Western Blot. Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	Albumin in Hepatocytes Derived from Human Embryonic Stem Cells. Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
	Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Simple Western
	Detection of Human Albumin by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Simple Western
	Detection of Human Albumin by Simple WesternTM. Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Entrez Gene IDs	213 (Human); 11657 (Mouse); 24186 (Rat)
Background	Albumin
background_content	Background: Albumin Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

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Citations

1. Extracellular vesicles from human liver stem cells restore argininosuccinate synthase deficiency
   Authors: MB Herrera Sa, S Previdi, S Bruno, V Fonsato, MC Deregibus, S Kholia, S Petrillo, E Tolosano, R Critelli, M Spada, R Romagnoli, M Salizzoni, C Tetta, G Camussi
   Stem Cell Res Ther, 2017;8(1):176.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Nucleic acid-based polymers effective against hepatitis B Virus infection in patients don't harbor immunostimulatory properties in primary isolated liver cells
   Authors: CI Real, M Werner, A Paul, G Gerken, JF Schlaak, A Vaillant, R Broering
   Sci Rep, 2017;7(0):43838.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood
   Nat Commun, 2016;7(0):13363.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh


4. Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model
   Stem Cells Dev, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC - Fresh


5. Differences in the Epigenetic Regulation of Cytochrome P450 Genes between Human Embryonic Stem Cell-Derived Hepatocytes and Primary Hepatocytes.
   Authors: Park H, Choi Y, Kim J, Chun H, Im I, Yoon S, Han Y, Song C, Kim H
   PLoS ONE, 2015;10(7):e0132992.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Fatty acid-binding proteins (FABPs) are intracellular carriers for Delta9-tetrahydrocannabinol (THC) and cannabidiol (CBD).
   Authors: Elmes M, Kaczocha M, Berger W, Leung K, Ralph B, Wang L, Sweeney J, Miyauchi J, Tsirka S, Ojima I, Deutsch D
   J Biol Chem, 2015;290(14):8711-21.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


7. Production of hepatocyte-like cells from human pluripotent stem cells.
   Authors: Hannan, Nicholas, Segeritz, Charis-P, Touboul, Thomas, Vallier, Ludovic
   Nat Protoc, 2013;8(2):430-7.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Identification of transcription factors for lineage-specific ESC differentiation.
   Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
   Stem Cell Reports, 2013;1(6):545-59.
   


9. Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease.
   Authors: Byun K, Bayarsaikhan E, Kim D, Kim CY, Mook-Jung I, Paek SH, Kim SU, Yamamoto T, Won MH, Song BJ, Park YM, Lee B
   PLoS ONE, 2012;7(5):e37917.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
    Authors: Hoang S, Liauw J, Choi M
    J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen

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