抗DLL4抗体(Anti-DLL4, Mouse, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse DLL4Ser28-Pro525Accession # Q9JI71
Source	Monoclonal Rat IgG2A Clone # 207822
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 100% cross-reactivity with recombinant human DLL4.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse DLL4 (Catalog # 1389-D4)
Immunohistochemistry	5-25 µg/mL	See below

Immunohistochemistry

DLL4 in Mouse Embryonic Heart. DLL4 was detected in immersion fixed frozen sections of mouse embryonic heart using Rat Anti-Human/Mouse DLL4 Monoclonal Antibody (Catalog # MAB1389) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing cardiomyocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

DLL4

background_content

Background: DLL4 Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

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Citations

1. Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development
   Elife, 2016;5(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. MEF2 transcription factors are key regulators of sprouting angiogenesis
   Authors: Sarah De Val
   Genes Dev., 2016;30(20):2297-2309.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Quantitative assessment of angiogenesis, perfused blood vessels and endothelial tip cells in the postnatal mouse brain.
   Authors: Walchli T, Mateos J, Weinman O, Babic D, Regli L, Hoerstrup S, Gerhardt H, Schwab M, Vogel J
   Nat Protoc, 2015;10(1):53-74.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. The alarmin IL-33 is a notch target in quiescent endothelial cells.
   Authors: Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G
   Am J Pathol, 2012;181(3):1099-111.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. KSHV-induced notch components render endothelial and mural cell characteristics and cell survival.
   Authors: Liu R, Li X, Tulpule A, Zhou Y, Scehnet JS, Zhang S, Lee JS, Chaudhary PM, Jung J, Gill PS
   Blood, 2010;115(4):887-95.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. CD8- DCs induce IL-12-independent Th1 differentiation through Delta 4 Notch-like ligand in response to bacterial LPS.
   Authors: Skokos D, Nussenzweig MC
   J. Exp. Med., 2007;204(7):1525-31.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB

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