抗Human BMP-7抗体(Anti-Human BMP-7 antibody)
掲載日情報：2019/10/21 現在Webページ番号：29300
Human BMP-7に対する抗体(Anti-Human BMP-7 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年04月26日 20時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||
|---|---|---|---|---|---|
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Anti-Human BMP-7 MAb (Clone 164311) |
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2 | |||
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Anti-Human BMP-7 MAb (Clone 164311) |
|
2 | |||
|
Anti-Human BMP-7 MAb (Clone 164311) |
|
2 | |||
[在庫・価格 ：2025年04月26日 20時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human BMP-7 MAb (Clone 164311)
文献数： 2
- 商品コード：MAB3541-100
- メーカー：RSD
- 包装：100μg
- 価格：¥101,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human BMP-7 MAb (Clone 164311)
文献数： 2
- 商品コード：MAB3541-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human BMP-7 MAb (Clone 164311)
文献数： 2
- 商品コード：MAB3541-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human BMP‑7Ser293-His431Accession # P18075 |
| Source | Monoclonal Mouse IgG2B Clone # 164311 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human BMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) BMP-6 is observed and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human BMP‑7 (Catalog # 354-BP) under non-reducing conditions only |
| Immunohistochemistry | 8-25 µg/mL | See below |
| Neutralization | Measured by its ability to neutralize BMP‑7-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Erlacher, L. et al. (1998) J. Bone Miner. Res. 13:383. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑7 and 50 µg/mL L-ascorbic acid. | |
| Neutralization | |
|---|---|
 | Alkaline Phosphatase Production Induced by BMP‑7 and Neutralization by Human BMP‑7 Antibody. Recombinant Human BMP‑7 (Catalog #354‑BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3541). The ND50 is typically 1.5-6.0 µg/mL in the presence of L-ascorbic acid (50 µg/mL). |
| Immunohistochemistry | |
 | BMP‑7 in Human Kidney. BMP‑7 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3541) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Related Product & Information
| Long Name | Bone Morphogenetic Protein 7 |
|---|---|
| Entrez Gene IDs | 655 (Human); 12162 (Mouse); 85272 (Rat) |
| Background | BMP-7 |
| background_content | Background: BMP-7 Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but often remains associated with the mature C-terminus. Based on in vivo and in vitro studies, BMP-7 has the potential to be secreted as a disulfide‑linked mature homodimer, or particularly as a heteromeric complex that consists of two propeptides noncovalently associated with a mature disulfide‑linked homodimer (5, 6). The presence of the propeptides in BMP-7 appears to stabilize the molecule and provide a docking mechanism for extracellular storage on molecules such as fibrillin-1 and -2 (5, 6). The propeptides themselves do not impart latency to the complex. BMP-7 binding to type II receptors rapidly displaces the prodomain:mature molecule interaction and has no effect on activity. But it is suggested that immobilized BMP-7 (via prodomain:fibrillin) is inactive, allowing for possible long-term storage of the molecule (6). BMP-7 interacts with the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 may also be processed into a disulfide‑linked heterodimer with either BMP-2 or BMP-4. Such complexes may show increased potency and range of activity compared to BMP-7 homodimers (7‑9). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13‑15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Regulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade.
Authors: Du Y, Xiao Q, Yip HK
Invest. Ophthalmol. Vis. Sci., 2010;51(7):3764-73.
Species: Mouse
Sample Type: Tissue Homogenates
Application: WB - Aggressive melanoma cells escape from BMP7-mediated autocrine growth inhibition through coordinated Noggin upregulation.
Authors: Hsu MY, Rovinsky SA, Lai CY, Qasem S, Liu X, How J, Engelhardt JF, Murphy GF
Lab. Invest., 2008;88(8):842-55.
Species: Human
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
J. Biol. Chem., 2008;283(20):13874-88.
Species: Human
Sample Type: Recombinant Protein
Application: WB - A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
J. Mol. Biol., 2008;381(4):1025-39.
Species: Human
Sample Type: Recombinant Protein
Application: ELISA Development - Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7.
Authors: Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT
J. Cell. Biochem., 2005;95(1):1-16.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: IP
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