抗PDGF Receptor α抗体(Anti-PDGF Receptor α, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Human osteosarcoma cell membrane extracts
Source	Monoclonal Mouse IgG1 Clone # PRa292
Purification	Protein A or G purified from ascites
Specificity	Detects human PDGF R alpha. Recognizes the PDGF receptor alpha -subunit; it does not recognize the PDGF receptor beta -subunit. Binds to the PDGF receptor alpha -subunit of primate species (human, monkey, baboon) and dog. Does not recognize rat or mouse receptors and its ability to bind to receptors from other species has not been tested.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	U‑118‑MG human glioblastoma/astrocytoma cell line
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human PDGF R alpha (Catalog # 322‑PR), see our available Western blot detection antibodies

目次に戻る

Related Product & Information

Background

PDGF R alpha

background_content

Background: PDGF R alpha PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

目次に戻る

Citations

1. PDGFRA alterations in cancer: characterization of a gain-of-function V536E transmembrane mutant as well as loss-of-function and passenger mutations.
   Authors: Velghe A, Van Cauwenberghe S, Polyansky A, Chand D, Montano-Almendras C, Charni S, Hallberg B, Essaghir A, Demoulin J
   Oncogene, 2014;33(20):2568-76.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Alveolar macrophages are critical for the inhibition of allergic asthma by mesenchymal stromal cells.
   Authors: Mathias, Louisa J, Khong, Sacha M, Spyroglou, Lisa, Payne, Natalie, Siatskas, Christop, Thorburn, Alison N, Boyd, Richard, Heng, Tracy S
   J Immunol, 2013;191(12):5914-24.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Mesenchymal stem cell abnormalities in patients with multiple myeloma.
   Authors: Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ
   Leuk. Lymphoma, 2007;48(10):2032-41.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Ubiquitinylation of Ig beta dictates the endocytic fate of the B cell antigen receptor.
   Authors: Zhang M, Veselits M, O'Neill S, Hou P, Reddi AL, Berlin I, Ikeda M, Nash PD, Longnecker R, Band H, Clark MR
   J. Immunol., 2007;179(7):4435-43.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
   Authors: Ball SG, Shuttleworth CA, Kielty CM
   J. Cell Biol., 2007;177(3):489-500.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


6. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
   Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
   Stem Cells, 2007;25(7):1737-45.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

目次に戻る