抗Nestin抗体(Anti-Nestin, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	NS0 mouse myeloma cell line transfected with human Nestin
Source	Monoclonal Mouse IgG1 Clone # 196908
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Nestin.

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Applications and Data

	Recommended Concentration	Sample
CyTOF-reported	This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin.

Immunocytochemistry
	Nestin in Human Neural Progenitor Cells. Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

Nestin

background_content

Background: Nestin Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

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Citations

1. Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease
   Authors: L Marrone, C Bus, D Schöndorf, JC Fitzgerald, M Kübler, B Schmid, P Reinhardt, L Reinhardt, M Deleidi, T Levin, A Meixner, B Klink, M Glatza, CJ Gloeckner, T Gasser, J Sternecker
   PLoS ONE, 2018;13(3):e0192497.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways
   Authors: F Wang, AY Wang, C Chesnelong, Y Yang, A Nabbi, S Thalappill, V Alekseev, K Riabowol
   Oncogene, 2018;37(3):286-301.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria
   Authors: F Hsu, S Spannl, C Ferguson, AA Hyman, RG Parton, M Zerial
   Elife, 2018;7(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


4. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
   Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
   Cell Rep, 2017;19(1):20-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo
   Authors: SS Jensen, SA Petterson, B Halle, C Aaberg-Jes, BW Kristensen
   BMC Cancer, 2017;17(1):178.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


6. Easy xeno-free and feeder-free method for isolating and growing limbal stromal and epithelial stem cells of the human cornea
   Authors: D Ghoubay-Be, C de Sousa, R Martos, G Latour, MC Schanne-Kl, E Dupin, V Borderie
   PLoS ONE, 2017;12(11):e0188398.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Induction of senescence in primary glioblastoma cells by serum and TGF?
   Authors: R Kumar, A Gont, TJ Perkins, JEL Hanson, IAJ Lorimer
   Sci Rep, 2017;7(1):2156.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
   Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
   Oncotarget, 2017;8(17):28074-28092.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Transferrin receptor-1 and ferritin heavy and light chains in astrocytic brain tumors: Expression and prognostic value
   Authors: AM Rosager, MD Sørensen, RH Dahlrot, S Hansen, DL Schonberg, JN Rich, JD Lathia, BW Kristensen
   PLoS ONE, 2017;12(8):e0182954.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC paraffin embedded


10. A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
    Authors: X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky
    PLoS ONE, 2017;12(4):e0174074.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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