抗Human PD-L2/B7-DC抗体(Anti-Human PD-L2/B7-DC antibody)
掲載日情報：2019/10/21 現在Webページ番号：29257
Human PD-L2/B7-DCに対する抗体(Anti-Human PD-L2/B7-DC )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月06日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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文献数 | ||
|---|---|---|---|---|---|
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Anti-Human PD-L2/B7-DC MAb (Clone 176611) |
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4 | |||
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Anti-Human PD-L2/B7-DC MAb (Clone 176611) |
|
4 | |||
|
Anti-Human PD-L2/B7-DC MAb (Clone 176611) |
|
4 | |||
[在庫・価格 ：2024年05月06日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human PD-L2/B7-DC MAb (Clone 176611)
文献数： 4
- 商品コード：MAB1224-100
- メーカー：RSD
- 包装：100μg
- 価格：¥103,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human PD-L2/B7-DC MAb (Clone 176611)
文献数： 4
- 商品コード：MAB1224-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human PD-L2/B7-DC MAb (Clone 176611)
文献数： 4
- 商品コード：MAB1224-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human PD-L2/B7-DCLeu20-Pro219 (predicted)Accession # Q9BQ51 |
| Source | Monoclonal Mouse IgG2B Clone # 176611 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human PD-L2/B7-DC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse PD-L2, recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H2, or rhB7-H3 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human PD-L2 Fc Chimera (Catalog # 1224-PL) |
| Immunohistochemistry | 0.5-25 µg/mL | See below |
| Immunohistochemistry | |
|---|---|
 | PD‑L2/B7‑DC in Human Lung Cancer Tissue. PD‑L2/B7‑DC was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human PD‑L2/B7‑DC Monoclonal Antibody (Catalog # MAB1224) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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Related Product & Information
| Background | PD-L2/B7-DC |
|---|---|
| background_content | Background: PD-L2/B7-DC T cells require a signal induced by the engagement of the T cell receptor and a “co‑stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell activation and tolerance. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co‑stimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (B7-DC) (1). B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned human PD-L2 cDNA encodes a 273 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 201 aa extracellular region containing one V-like and one C-like Ig domain, a 24 aa transmembrane region, and a 28 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 70% aa sequence identity (2). PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. The other identified ligand is PD-L1. Human PD-L1 and PD-L2 share approximately 41% aa sequence identity and have similar functions. PD-L2 is broadly expressed in tissues. Highest expression was detected by Northern blot analysis in heart, placenta, liver, pancreas, spleen, and lymph node. Lower amounts of expression were observed in lung, smooth muscle, and thymus. Expression of PD-L2 on antigen presenting cell has been examined in detail. Resting B cells, monocytes and dendritic cells do not express PD-L2, expression however can be induced by LPS or BCR activation in B cells, INF-gamma treatment in monocytes, or LPS plus IFN-gamma treatment of dendritic cells. PD-L2 expression is also up regulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co‑stimulatory function for the PD-L2 on resting T cells activated with sub-optimal TCR signals has also been reported (3). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells
Authors: MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields
Nat Commun, 2018;9(1):948.
Species: Mouse
Sample Type: Whole Cells
Application: Flow - PD-L1 expression and presence of TILs in small intestinal neuroendocrine tumours
Authors: A Lamarca, D Nonaka, W Breitwiese, G Ashton, J Barriuso, MG McNamara, S Moghadam, J Rogan, W Mansoor, RA Hubner, C Clark, B Chakrabart, JW Valle
Oncotarget, 2018;9(19):14922-14938.
Species: Human
Sample Type: Whole Cells
Application: ICC - PD-L1 is a Prognostic Biomarker in Resected NSCLC Patients with Moderate/high Smoking History and Elevated Serum SCCA Level
Authors: L Cao, X Wang, S Li, Q Zhi, Y Wang, L Wang, K Li, R Jiang
J Cancer, 2017;8(16):3251-3260.
Species: Human
Sample Type: Whole Tissue
Application: IHC-P - Clinicopathologic analysis of programmed cell death-1 and programmed cell death-ligand 1 and 2 expressions in pulmonary adenocarcinoma: comparison with histology and driver oncogenic alteration status.
Authors: Koh J, Go H, Keam B, Kim M, Nam S, Kim T, Lee S, Min H, Kim Y, Kim D, Jeon Y, Chung D
Mod Pathol, 2015;28(9):1154-66.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Not Specified - IFN-gamma generates maturation-arrested dendritic cells that induce T cell hyporesponsiveness independent of Foxp3+ T-regulatory cell generation.
Authors: Rojas D, Krishnan R,
Immunol. Lett., 2010;132(1):31-7.
Species: Human
Sample Type: Whole Cells
Application: Flow - Expression of B7-H1 on gastric epithelial cells: its potential role in regulating T cells during Helicobacter pylori infection.
Authors: Das S, Suarez G, Beswick EJ, Sierra JC, Graham DY, Reyes VE
J. Immunol., 2006;176(5):3000-9.
Species: Human
Sample Type: Cell Lysates
Application: WB
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