抗Human DC-SIGN+DC-SIGNR抗体(Anti-Human DC-SIGN+DC-SIGNR antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNRAccession # Q9H2X3
Source	Monoclonal Mouse IgG2A Clone # 120612
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Recognizes both human DC-SIGN and human DC-SIGNR on transfected cells. Does not react with parental mouse cells or irrelevant transfectants.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of DC‑SIGN+DC‑SIGNR in Human DC‑SIGN or DC-SIGNR Transfected 3T3 Mouse Cell Line by Flow Cytometry. Human DC‑SIGN and DC‑SIGNR transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGN+DC‑SIGNR Monoclonal Antibody (Catalog # MAB1621, filled histo­grams) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Flow Cytometry
	Detection of DC‑SIGN+DC‑SIGNR in Human Monocyte Derived Dendritic Cells by Flow Cytometry. Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC‑SIGN+ DC‑SIGNR Monoclonal Antibody (Catalog # MAB1621), followed by PE-conjugated anti-mouse secondary antibody (Catalog # F0102B) and Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F).Quadrant markers were set based on isotype control antibody staining (Catalog # MAB003).
Immunohistochemistry
	DC‑SIGN+DC‑SIGNR in Human Lymphoma. DC‑SIGN+DC‑SIGNR was detected in immersion fixed paraffin-embedded sections of human lymphoma using 25 µg/mL Mouse Anti-Human DC‑SIGN+DC‑SIGNR Monoclonal Antibody (Catalog # MAB1621) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background

DC-SIGN+DC-SIGNR

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Background: DC-SIGN+DC-SIGNR DC-SIGN (Dendritic Cell- Specific ICAM-3 Grabbing Non-Integrin) has been shown to play an important role in regulating dendritic cell (DC) and T cell interactions, including antigen presentation to T cells and enhancement of transinfection of CD4+ T cells by HIV-1 (1, 2). Efforts to identify additional type II membrane proteins resulted in the isolation of a molecule related in sequence to DC-SIGN known as DC-SIGNR (DC-SIGN Related) (3, 4). DC-SIGNR shares 73 - 80% amino acid homology with DC-SIGN and is located on human chromosome 19p13.3. Its structure is similar to DC-SIGN and therefore binds mannose residues in a calcium dependent fashion, including ICAM-3 and HIV-1 gp120 (5). DC-SIGNR, also known as L-SIGN (Liver/Lymph node-Specific ICAM-3-Grabbing Non-integrin) and DC-SIGNR, is polymorphic since allelic variations of the exon 4 encoded sequence have been isolated (5). This is further supported by a study demonstrating the ability to isolate a large repertoire of DC-SIGNR transcripts largely the result of alternative splicing of the 7 coding exons (6). L-SIGN/DC-SIGNR is primarily transcribed in the liver and lymph nodes but not in monocyte derived DC (5). Expression of L-SIGN/DC-SIGNR is restricted to endothelial cells derived from liver sinusoids, lymph nodes sinuses and capillaries (7) although variable expression in placenta and some monocytic cell lines has also been reported, including both membrane and soluble isoforms of the protein (6). Expression of DC-SIGN is induced during the in-vitro generation of DC from either monocytes or bone marrow progenitors, with maximal surface expression at day 7 of culture (1). Immature DC in the skin and mature DC in the tonsil have been demonstrated to express DC-SIGN (8). Analysis of various tissues and cell lines suggests that DC-SIGN expression is restricted to DC (1) although a more recent report finds evidence of expression in placenta, resting monocytes and monocytic cell lines (6). This discrepancy may be partially related to the multiple isoforms of DC-SIGN transcripts, including both membrane and soluble forms, as well as exon splice variants reported in the latter study (6).

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Citations

1. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication
   Authors: KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse
   Sci Rep, 2017;7(0):41389.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Uukuniemi virus as a tick-borne virus model
   J Virol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Binding of HIV-1 gp120 to DC-SIGN promotes ASK-1-dependent activation-induced apoptosis of human dendritic cells.
   Authors: Chen Y, Hwang S, Chan V, Chung N, Wang S, Li Z, Ma J, Lin C, Hsieh Y, Chang K, Kung S, Wu Y, Chu C, Tai H, Gao G, Zheng B, Yokoyama K, Austyn J, Lin C
   PLoS Pathog, 2013;9(1):e1003100.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Vaccine protection by live, attenuated simian immunodeficiency virus in the absence of high-titer antibody responses and high-frequency cellular immune responses measurable in the periphery.
   Authors: Mansfield K, Lang SM, Gauduin MC, Sanford HB, Lifson JD, Johnson RP, Desrosiers RC
   J. Virol., 2008;82(8):4135-48.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Cells
   Application: ICC


5. SIV-associated myocarditis: viral and cellular correlates of inflammation severity.
   Authors: Yearley JH, Pearson C, Carville A, Shannon RP, Mansfield KG
   AIDS Res. Hum. Retroviruses, 2006;22(6):529-40.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes.
   Authors: Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M
   AIDS Res. Hum. Retroviruses, 2006;22(11):1152-61.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN.
   Authors: Wu L, Bashirova AA, Martin TD, Villamide L, Mehlhop E, Chertov AO, Unutmaz D, Pope M, Carrington M, KewalRamani VN
   Proc. Natl. Acad. Sci. U.S.A., 2002;99(3):1568-73.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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