抗Human IGF-I R Phycoerythrin抗体(Anti-Human IGF-I R Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I RGlu31-Asn932Accession # P08069
Source	Monoclonal Mouse IgG1 Clone # 33255
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IGF-I R in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL-9 R, and rhTGF-beta  RII.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of IGF‑I R in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human IGF‑I R PE‑conjugated Monoclonal Antibody (Catalog # FAB391P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Insulin-like Growth Factor I Receptor
Background	IGF-I R
background_content	Background: IGF-I R Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

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Citations

1. Quantification of IGF-1 Receptor May Be Useful in Diagnosing Polycythemia Vera-Suggestion to Be Added to Be One of the Minor Criterion
   PLoS ONE, 2016;11(11):e0165299.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
   Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
   Br J Cancer, 2014;110(12):2865-73.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
   Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
   PLoS ONE, 2014;9(1):e84417.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Clinical and functional characterization of a patient carrying a compound heterozygous pericentrin mutation and a heterozygous IGF1 receptor mutation.
   Authors: Muller E, Dunstheimer D, Klammt J, Friebe D, Kiess W, Kratzsch J, Kruis T, Laue S, Pfaffle R, Wallborn T, Heidemann PH
   PLoS ONE, 2012;7(5):e38220.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Insulin-like growth factor I (IGF-I) stimulates proliferation but also increases caspase-3 activity, Annexin-V binding, and DNA-fragmentation in human MG63 osteosarcoma cells: co-activation of pro- and anti-apoptotic pathways by IGF-I.
   Authors: Raile K, Hille R, Laue S, Schulz A, Pfeifer G, Horn F, Kiess W
   Horm. Metab. Res., 2003;35(11):786-93.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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