抗Human VEGF R2/KDR Allophycocyanin抗体(Anti-Human VEGF R2/KDR Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1Ala20-Glu764Accession # P35968
Source	Monoclonal Mouse IgG1 Clone # 89106
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk‑1 APC‑conjugated Mono­clonal Antibody (Catalog # FAB357A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

VEGF R2/KDR/Flk-1

background_content

Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

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Citations

1. Vascular CXCR4 expression promotes vessel sprouting and sensitivity to sorafenib treatment in hepatocellular carcinoma
   Authors: J Xu, J Liang, YM Meng, J Yan, XJ Yu, CQ Liu, L Xu, SM Zhuang, L Zheng
   Clin. Cancer Res, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Endothelial progenitor cells and plaque burden in stented coronary artery segments: an optical coherence tomography study six months after elective PCI
   Authors: S Otto, K Nitsche, C Jung, A Kryvanos, A Zhylka, K Heitkamp, JL Gutiérrez-, B Goebel, PC Schulze, HR Figulla, TC Poerner
   BMC Cardiovasc Disord, 2017;17(1):103.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. The distribution and potential prognostic value of SMAD protein expression in chronic lymphocytic leukemia
   Authors: M Witkowska, A Majchrzak, B Cebula-Obr, E Wawrzyniak, T Robak, P Smolewski
   Tumour Biol, 2017;39(3):1010428317694.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
   Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
   PLoS ONE, 2016;11(4):e0152993.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker
   Nat. Biotechnol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Kinetics of circulating endothelial progenitor cells in patients undergoing carotid artery surgery
   Authors: Günay Kalender
   Ther Clin Risk Manag, 2016;12(0):1841-1847.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
   Authors: Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G
   PLoS ONE, 2014;9(1):e84179.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Circulating autoantibodies to endothelial progenitor cells: binding characteristics and association with risk factors for atherosclerosis.
   Authors: George J, Matucci-Cerinic M, Bar I, Shimoni S
   PLoS ONE, 2014;9(6):e97836.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Ovine carotid artery-derived cells as an optimized supportive cell layer in 2-D capillary network assays.
   Authors: Weinandy, Stefan, Babczyk, Patrick, Dreier, Agnieszk, Unger, Ronald E, Flanagan, Thomas C, Kirkpatrick, C James, Zenke, Martin, Klee, Doris, Jockenhoevel, Stefan
   PLoS ONE, 2014;9(3):e91664.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. E-selectin mediated adhesion and migration of endothelial colony forming cells is enhanced by SDF-1alpha/CXCR4.
    Authors: Sun J, Li Y, Graziani G, Filion L, Allan D
    PLoS ONE, 2013;8(4):e60890.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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