抗IL-1α抗体(Anti-IL-1α, Human, Mouse-Mono, FITC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	E. coli-derived recombinant human IL‑1 alpha /IL‑1F1Ser113-Ala271Accession # P01583
Source	Monoclonal Mouse IgG1 Clone # 3405
Purification	Protein A or G purified from ascites
Specificity	Detects human IL‑1 alpha /IL‑1F1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-1 alpha, recombinant rat IL-1 alpha, recombinant cotton rat IL-1 alpha, recombinant human (rh) IL-1 beta or rhIL-18 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of IL‑1 alpha /IL‑1F1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS for 24 hours were stained with Mouse Anti-Human IL‑1 alpha /IL‑1F1 Membrane Form Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB200F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Interleukin 1 alpha
Background	IL-1 alpha/IL-1F1
background_content	Background: IL-1 alpha/IL-1F1 Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia). IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

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Citations

1. The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence
   Authors: SM Ong, E Hadadi, TM Dang, WH Yeap, CT Tan, TP Ng, A Larbi, SC Wong
   Cell Death Dis, 2018;9(3):266.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
   Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
   Eur J Immunol, 2015;45(4):1092-102.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation.
   Authors: Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, Curran S, Davalos A, Wilson-Edell K, Liu S, Limbad C, Demaria M, Li P, Hubbard G, Ikeno Y, Javors M, Desprez P, Benz C, Kapahi P, Nelson P, Campisi J
   Nat Cell Biol, 2015;17(8):1049-61.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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