抗p53抗体(Anti-p53, Human/Mouse/Rat, Goat-Poly, HRP antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	HRP (Horseradish Peroxidase)
Immunogen	E. coli-derived recombinant human p53Asp7-Asp393Accession # P04637
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat p53 in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1:5000 dilution	See below

Western Blot

Detection of Human p53 by Western Blot. Western blot shows lysates of CEM human T‑lymphoblastoid cell line and MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 10 Gy ionizing radiation (IR) for 1 hour. PVDF membrane was probed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # HAF1355). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
Background	p53
background_content	Background: p53 The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

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Citations

1. The MBT repeats of L3MBTL1 link SET8-mediated p53 methylation at lysine 382 to target gene repression.
   Authors: West LE, Roy S, Lachmi-Weiner K
   J. Biol. Chem., 2010;285(48):37725-32.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. KRAB-type zinc-finger protein Apak specifically regulates p53-dependent apoptosis.
   Authors: Tian C, Xing G, Xie P, Lu K, Nie J, Wang J, Li L, Gao M, Zhang L, He F
   Nat. Cell Biol., 2009;11(5):580-91.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Role for 53BP1 Tudor domain recognition of p53 dimethylated at lysine 382 in DNA damage signaling.
   Authors: Kachirskaia I, Shi X, Yamaguchi H, Tanoue K, Wen H, Wang EW, Appella E, Gozani O
   J. Biol. Chem., 2008;283(50):34660-6.
   Species: N/A
   Sample Type: Recombinant Protein
   Application: WB


4. Histone methyltransferase protein SETD2 interacts with p53 and selectively regulates its downstream genes.
   Authors: Xie P, Tian C, An L, Nie J, Lu K, Xing G, Zhang L, He F
   Cell. Signal., 2008;20(9):1671-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Arginine methylation of hnRNP K enhances p53 transcriptional activity.
   Authors: Chen Y, Zhou X, Liu N, Wang C, Zhang L, Mo W, Hu G
   FEBS Lett., 2008;582(12):1761-5.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. SVH-B interacts directly with p53 and suppresses the transcriptional activity of p53.
   Authors: Zhou X, Yang G, Huang R, Chen X, Hu G
   FEBS Lett., 2007;581(25):4943-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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