抗p53抗体(Anti-p53, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Biotin
Immunogen	E. coli-derived recombinant human p53Asp7-Asp393Accession # P04637
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat p53.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human p53 Recombinant Mouse p53 Recombinant Rat p53

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Related Product & Information

Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
Background	p53
background_content	Background: p53 p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis including cell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.

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Citations

1. Scintillation proximity assay for total p53 protein as an alternative to ELISA.
   Authors: Oberlander S, Xie T, Chandrachud U
   J. Immunol. Methods, 2010;360(1):173-7.
   Species: Human
   Sample Type: Cell Lysates
   Application: scintillation proximity assay


2. In vitro analysis of DNA-protein interactions by proximity ligation.
   Authors: Gustafsdottir SM, Schlingemann J, Rada-Iglesias A, Schallmeiner E, Kamali-Moghaddam M, Wadelius C, Landegren U
   Proc. Natl. Acad. Sci. U.S.A., 2007;104(9):3067-72.
   Species: Human
   Sample Type: Cell Lysates
   Application: Proximity Ligation Assay

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