抗Human E-Selectin/CD62E抗体(Anti-Human E-Selectin/CD62E antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Activated HUVEC human umbilical vein endothelial cells
Source	Monoclonal Mouse IgG1 Clone # BBIG-E1
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	This antibody binds to CHO cells transfected with human E-Selectin. It does not bind to COS cells transfected with human PECAM‑1, P‑Selectin, L‑Selectin, VCAM‑1, or ICAM‑1.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunoprecipitation	Pigott, R. et al. (1991) J. Immunol. 147:130.
Adhesion Blockade	The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human E‑Selectin (Catalog # ADP1, 2 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Immunofluorescence	Pigott, R. et al. (1991) J. Immunol. 147:130.

Immunocytochemistry

E‑Selectin/CD62E in HUVECs. E‑Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with Recombinant Human TNF-alpha (Catalog # 210-TA) using Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA26) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Entrez Gene IDs	6401 (Human); 20339 (Mouse); 25544 (Rat)
Background	E-Selectin/CD62E
background_content	Background: E-Selectin/CD62E E-Selectin, also known as ELAM-1, is a transmembrane glycoprotein that is transiently expressed on activated vascular endothelial cells. E-Selectin binds to sialyated, fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells.

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Citations

1. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium
   Authors: FW Lam, Q Da, B Guillory, MA Cruz
   J. Immunol., 2018;0(0):.
   Species: Human
   Sample Type: Recombinant Protein
   Application: Blocking


2. A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells
   Stem Cells, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63.
   Authors: Poeter M, Brandherm I, Rossaint J, Rosso G, Shahin V, Skryabin B, Zarbock A, Gerke V, Rescher U
   Nat Commun, 2014;5(0):3738.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
   Authors: Pezeshkian, Bahareh, Donnelly, Christop, Tamburo, Kelley, Geddes, Timothy, Madlambayan, Gerard J
   PLoS ONE, 2013;8(4):e60823.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


5. The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
   Authors: Fitau J, Boulday G, Coulon F, Quillard T, Charreau B
   J. Biol. Chem., 2006;281(29):20148-59.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism.
   Authors: Lawson C, Ainsworth M, Yacoub M, Rose M
   J. Immunol., 1999;162(5):2990-6.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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