抗VCAM-1抗体(Anti-VCAM-1, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Activated HUVEC human umbilical vein endothelial cells
Source	Monoclonal Mouse IgG1 Clone # BBIG-V1
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human VCAM‑1/CD106 (Catalog # 809-VR) under non-reducing conditions only
Immunoprecipitation	10 µg/mL	Radiolabelled activated HUVEC human umbilical vein endothelial cells, see our available Western blot detection antibodies
Adhesion Blockade	The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human VCAM-1/CD106 (Catalog # ADP5, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 30 µg/mL of the antibody.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

VCAM‑1/CD106 in HUVEC Cells. VCAM‑1/CD106 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # BBA5) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue.

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Related Product & Information

Long Name	Vascular Cell Adhesion Molecule 1
Entrez Gene IDs	7412 (Human); 22329 (Mouse)
Background	VCAM-1/CD106
background_content	Background: VCAM-1/CD106 Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

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Citations

1. Engineering a multicellular vascular niche to model hematopoietic cell trafficking
   Authors: SS Kotha, BJ Hayes, KT Phong, MA Redd, K Bomsztyk, A Ramakrishn, B Torok-Stor, Y Zheng
   Stem Cell Res Ther, 2018;9(1):77.
   Application: Neut


2. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium
   Authors: FW Lam, Q Da, B Guillory, MA Cruz
   J. Immunol., 2018;0(0):.
   Species: Human
   Sample Type: Recombinant Protein
   Application: Blocking


3. Experimental identification and computational characterization of a novel extracellular metalloproteinase produced by Clostridium sordellii
   Authors: MJ Aldape, A Tao, DD Heeney, ER McIndoo, JM French, D Xu
   RSC Adv, 2017;7(23):13928-13938.
   Application: WB


4. Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate
   Authors: PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley
   J. Leukoc. Biol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. p38 MAP kinase inhibitor suppresses transforming growth factor-beta2-induced type 1 collagen production in trabecular meshwork cells.
   Authors: Inoue-Mochita M, Inoue T, Fujimoto T, Kameda T, Awai-Kasaoka N, Ohtsu N, Kimoto K, Tanihara H
   PLoS ONE, 2015;10(3):e0120774.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
   Authors: Roe, Kelsey, Orillo, Beverly, Verma, Saguna
   PLoS ONE, 2014;9(7):e102598.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
   Authors: Park, Su-Jung, Kim, Ki-Jo, Kim, Wan-Uk, Cho, Chul-Soo
   J Immunol, 2014;192(7):3003-10.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
   Authors: Lauridsen H, Pober J, Gonzalez A
   FASEB J, 2014;28(3):1166-80.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece.
   Authors: Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P
   PLoS ONE, 2014;9(4):e94860.
   Species: Human
   Sample Type: Whole Cells
   Application: ELISA Capture


10. Uveal melanoma cells utilize a novel route for transendothelial migration.
    Authors: Onken, Michael, Li, Jinmei, Cooper, John A
    PLoS ONE, 2014;9(12):e115472.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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