抗ICAM-1抗体(Anti-ICAM-1, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Activated HUVEC human umbilical vein endothelial cells
Source	Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	This antibody was selected for its ability to stain human ICAM-1/CD54 transfected COS cells. It does not stain COS cells transfected with human E-Selectin, VCAM-1, or PECAM-1.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human ICAM‑1/CD54 Fc Chimera (Catalog # 720-IC) under non-reducing conditions only
Immunoprecipitation	Davies, M.E., et al. (1991) Connect. Tissue Res. 26:207.
Adhesion Blockade	The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC,12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

ICAM‑1/CD54 in HUVEC Cells. ICAM‑1/CD54 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Mouse Anti-HumanICAM‑1/CD54 Monoclonal Antibody (Catalog # BBA3) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Intercellular Adhesion Molecule 1
Entrez Gene IDs	3383 (Human); 15894 (Mouse); 25464 (Rat)
Background	ICAM-1/CD54
background_content	Background: ICAM-1/CD54 ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

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Citations

1. Inflammation-Sensitive Myosin-X Functionally Supports Leukocyte Extravasation by Cdc42-Mediated ICAM-1-Rich Endothelial Filopodia Formation
   Authors: J Kroon, A Schaefer, J van Rijsse, M Hoogenboez, F van Alphen, P Hordijk, ESG Stroes, S Strömblad, J van Rheene, JD van Buul
   J. Immunol., 2018;0(0):.
   Application: Bead coating


2. A quantitative method for screening and identifying molecular targets for nanomedicine
   Authors: P Guo, J Yang, DR Bielenberg, D Dillon, D Zurakowski, MA Moses, DT Auguste
   J Control Release, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro
   Authors: A Schaefer, TJ van Duijn, J Majolee, K Burridge, PL Hordijk
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


4. A possible role for neutrophils in allergic rhinitis revealed after cellular subclassification
   Authors: J Arebro, S Ekstedt, E Hjalmarsso, O Winqvist, S Kumlien Ge, LO Cardell
   Sci Rep, 2017;7(0):43568.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. 3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells
   Authors: Minu Karthika Ganesan
   Tissue Eng Part C Methods, 2016;0(0):.
   Species: Human
   Sample Type: Complex Sample Type
   Application: ICC


6. Role of cortactin homolog HS1 in transendothelial migration of natural killer cells.
   Authors: Mukherjee S, Kim J, Mooren O, Shahan S, Cohan M, Cooper J
   PLoS ONE, 2015;10(2):e0118153.
   Species: Human
   Sample Type: Whole Cells
   Application: IF


7. C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation.
   Authors: Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
   Sci Rep, 2015;5(0):9275.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Enzyme-free passage of human pluripotent stem cells by controlling divalent cations.
   Authors: Ohnuma, Kiyoshi, Fujiki, Ayaka, Yanagihara, Kana, Tachikawa, Saoko, Hayashi, Yohei, Ito, Yuzuru, Onuma, Yasuko, Chan, Techuan, Michiue, Tatsuo, Furue, Miho K, Asashima, Makoto
   Sci Rep, 2014;4(0):4646.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC


9. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
   Authors: Park, Su-Jung, Kim, Ki-Jo, Kim, Wan-Uk, Cho, Chul-Soo
   J Immunol, 2014;192(7):3003-10.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Inflammation converts human mesoangioblasts into targets of alloreactive immune responses: implications for allogeneic cell therapy of DMD.
    Authors: Noviello M, Tedesco F, Bondanza A, Tonlorenzi R, Rosaria Carbone M, Gerli M, Marktel S, Napolitano S, Cicalese M, Ciceri F, Peretti G, Cossu G, Bonini C
    Mol Ther, 2014;22(7):1342-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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