抗E-Selectin/P-Selectin抗体(Anti-E-Selectin/P-Selectin, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Activated HUVEC human umbilical vein endothelial cells
Source	Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunoprecipitation	Pigott, R. et al. (1991) J. Immunol. 147:130.
Adhesion Blockade	The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells. E-Selectin (CD62E)/P-Selectin (CD62P) was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210‑TA‑010) using Mouse Anti-Human E-Selectin (CD62E)/P-Selectin (CD62P) Monoclonal Antibody (Catalog # BBA1) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background	E-Selectin (CD62E)/P-Selectin (CD62P)
background_content	Background: E-Selectin (CD62E)/P-Selectin (CD62P) E-Selectin and P-Selectin are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C‑type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

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Citations

1. Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction
   Authors: JD Belcher, C Chen, J Nguyen, F Abdulla, P Zhang, H Nguyen, P Nguyen, T Killeen, SM Miescher, N Brinkman, KA Nath, CJ Steer, GM Vercellott
   PLoS ONE, 2018;13(4):e0196455.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Self-medication by orang-utans (Pongo pygmaeus) using bioactive properties of Dracaena cantleyi
   Authors: HC Morrogh-Be, I Foitová, Z Yeen, P Wilkin, R de Martin, L Rárová, K Doležal, W Nurcahyo, M Olšanský
   Sci Rep, 2017;7(1):16653.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. SDF-1?-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia
   Sci Rep, 2016;6(0):34416.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells
   PLoS ONE, 2016;11(12):e0167361.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
   Authors: Roe, Kelsey, Orillo, Beverly, Verma, Saguna
   PLoS ONE, 2014;9(7):e102598.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Functional diversity of human vaginal APC subsets in directing T-cell responses.
   Authors: Duluc, D, Gannevat, J, Anguiano, E, Zurawski, S, Carley, M, Boreham, M, Stecher, J, Dullaers, M, Banchereau, J, Oh, S
   Mucosal Immunol, 2013;6(3):626-38.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: Flow


7. Activation of primary porcine endothelial cells induces release of porcine endogenous retroviruses.
   Authors: Cunningham DA, Dos Santos Cruz GJ, Fernandez-Suarez XM, Whittam AJ, Herring C, Copeman L, Richards A, Langford GA
   Transplantation, 2004;77(7):1071-9.
   Species: Porcine
   Sample Type: Whole Cells
   Application: Flow

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