抗Nanog抗体(Anti-Nanog, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human NanogTrp153-Val305Accession # ABZ92376
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human Nanog by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.
Chromatin Immunoprecipitation (ChIP)
	Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.
Immunocytochemistry
	Nanog in BG01V Human Stem Cells. Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	Nanog in BG01V Human Stem Cells-derived Embryoid Body. Nanog was detected in immersion fixed BG01V human embryonic stem cells-derived embryoid body using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	Nanog in ADLF1 and FAB2 Stem Cell Lines. Nanog was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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Related Product & Information

Background	Nanog
background_content	Background: Nanog Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).

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Citations

1. TFAP2C regulates transcription in human naive pluripotency by opening enhancers
   Authors: WA Pastor, W Liu, D Chen, J Ho, R Kim, TJ Hunt, A Lukianchik, X Liu, JM Polo, SE Jacobsen, AT Clark
   Nat. Cell Biol., 2018;20(5):553-564.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Generation of a human induced pluripotent stem cell line from a patient with a rare A673T variant in amyloid precursor protein gene that reduces the risk for Alzheimer's disease
   Authors: Š Lehtonen, I Höytyläine, J Voutilaine, TM Sonninen, J Kuusisto, M Laakso, RH Hämäläinen, M Oksanen, J Koistinaho
   Stem Cell Res, 2018;30(0):96-99.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions
   Authors: RK Jangid, A Kelkar, VY Muley, S Galande
   BMC Genomics, 2018;19(1):313.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Biological characterization of human amniotic epithelial cells in a serum-free system and their safety evaluation
   Authors: PJ Yang, WX Yuan, J Liu, JY Li, B Tan, C Qiu, XL Zhu, C Qiu, DM Lai, LH Guo, LY Yu
   Acta Pharmacol. Sin., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. High-efficiency RNA-based reprogramming of human primary fibroblasts
   Authors: I Kogut, SM McCarthy, M Pavlova, DP Astling, X Chen, A Jakimenko, KL Jones, A Getahun, JC Cambier, AMG Pasmooij, MF Jonkman, DR Roop, G Bilousova
   Nat Commun, 2018;9(1):745.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo
   Authors: OM Russell, I Fruh, PK Rai, D Marcellin, T Doll, A Reeve, M Germain, J Bastien, KA Rygiel, R Cerino, AW Sailer, M Lako, RW Taylor, M Mueller, RN Lightowler, DM Turnbull, SB Helliwell
   Sci Rep, 2018;8(1):1799.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9
   Authors: Y Xu, Y Wu, D Guo, G Gao, K Lai, F Yang, K Wang, H Wu, L Lai, J Li, K Xu, YX Li
   Stem Cell Res, 2017;22(0):29-32.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders
   Authors: C Lorenz, P Lesimple, R Bukowiecki, A Zink, G Inak, B Mlody, M Singh, M Semtner, N Mah, K Aur‚, M Leong, O Zabiegalov, EM Lyras, V Pfiffer, B Fauler, J Eichhorst, B Wiesner, N Huebner, J Priller, T Mielke, D Meierhofer, Z Izsv k, JC Meier, F Bouillaud, J Adjaye, M Schuelke, EE Wanker, A LombŠs, A Prigione
   Cell Stem Cell, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration
   Authors: JS Saini, B Corneo, JD Miller, TR Kiehl, Q Wang, NC Boles, TA Blenkinsop, JH Stern, S Temple
   Cell Stem Cell, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
    Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
    Nat Protoc, 2017;12(4):814-827.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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