抗CD32a抗体(Anti-CD32a, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Fc gamma  RIIA/CD32aAla36-Ile218Accession # AAA35827
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Fc gamma  RIIA/CD32a in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Fc gamma  RIIB and rhFc gamma  RIIIB is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Fc gamma  RIIA/CD32a (Catalog # 1330-CD)
Flow Cytometry	2.5 µg/106 cells	Human peripheral blood granulocytes
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of human IgG to immobilized Recombinant Human Fc gamma RIIA/CD32a (Catalog # 1330-CD) coated at 1 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

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Related Product & Information

Long Name	Fc gamma Receptor II A
Entrez Gene IDs	2212 (Human); 14130 (Mouse); 116591 (Rat); 102143568 (Cynomolgus Monkey)
Background	Fc gamma RIIA/CD32a
background_content	Background: Fc gamma RIIA/CD32a Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is ahigh-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC). Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified (1 - 3). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) (4, 5). Two allelic variants (R167 and H167) of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The H167 allele has been found to have a protective effect against lupus nephritis.

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Citations

1. Antibody-dependent and -independent uptake of HBsAg across human leukocyte subsets is similar between individuals with chronic hepatitis B Virus infection and healthy donors
   Authors: Hugo Tharinger
   J. Viral Hepat, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Viremic HIV Controllers Exhibit High Plasmacytoid Dendritic Cell-Reactive Opsonophagocytic IgG Antibody Responses against HIV-1 p24 Associated with Greater Antibody Isotype Diversification.
   Authors: Tjiam M, Taylor J, Morshidi M, Sariputra L, Burrows S, Martin J, Deeks S, Tan D, Lee S, Fernandez S, French M
   J Immunol, 2015;194(11):5320-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. FcgammaRI mediates serum amyloid P inhibition of fibrocyte differentiation.
   Authors: Crawford J, Pilling D, Gomer R
   J Leukoc Biol, 2012;92(4):699-711.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Nonfucosylated anti-CD20 antibody potentially induces apoptosis in lymphoma cells through enhanced interaction with FcgammaRIIIb on neutrophils.
   Authors: Nakagawa T, Natsume A, Satoh M, Niwa R
   Leuk. Res., 2010;34(5):666-71.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. The ubiquitin ligase c-Cbl down-regulates FcgammaRIIa activation in human neutrophils.
   Authors: Marois L, Vaillancourt M, Marois S, Proulx S, Pare G, Rollet-Labelle E, Naccache PH
   J. Immunol., 2009;182(4):2374-84.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Association of FcgammaRIIa (CD32a) with lipid rafts regulates ligand binding activity.
   Authors: Bournazos S, Hart SP, Chamberlain LH, Glennie MJ, Dransfield I
   J. Immunol., 2009;182(12):8026-36.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
   Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
   J. Leukoc. Biol., 2008;84(4):1109-19.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
   Authors: Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M
   Atherosclerosis, 2007;196(1):129-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. C-reactive protein impairs human CD14+ monocyte-derived dendritic cell differentiation, maturation and function.
   Authors: Zhang R, Becnel L, Li M, Chen C, Yao Q
   Eur. J. Immunol., 2006;36(11):2993-3006.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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